概述

  • 产品名称Anti-Smad4抗体
    参阅全部 Smad4 一抗
  • 描述
    兔多克隆抗体to Smad4
  • 经测试应用适用于: WBmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Horse, Cow, Dog, Pig, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Smad4.

  • 阳性对照
    • WB: HeLa, A431, Jurkat, A549, HepG2, THP1 and Ramos whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab110175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 60 kDa).
  • 应用说明Is unsuitable for ICC/IF.
  • 靶标

    • 功能Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for syngernistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor.
    • 疾病相关Defects in SMAD4 are a cause of pancreatic cancer (PNCA) [MIM:260350].
      Defects in SMAD4 are a cause of juvenile polyposis syndrome (JPS) [MIM:174900]; also known as juvenile intestinal polyposis (JIP). JPS is an autosomal dominant gastrointestinal hamartomatous polyposis syndrome in which patients are at risk for developing gastrointestinal cancers. The lesions are typified by a smooth histological appearance, predominant stroma, cystic spaces and lack of a smooth muscle core. Multiple juvenile polyps usually occur in a number of Mendelian disorders. Sometimes, these polyps occur without associated features as in JPS; here, polyps tend to occur in the large bowel and are associated with an increased risk of colon and other gastrointestinal cancers.
      Defects in SMAD4 are a cause of juvenile polyposis/hereditary hemorrhagic telangiectasia syndrome (JP/HHT) [MIM:175050]. JP/HHT syndrome phenotype consists of the coexistence of juvenile polyposis (JIP) and hereditary hemorrhagic telangiectasia (HHT) [MIM:187300] in a single individual. JIP and HHT are autosomal dominant disorders with distinct and non-overlapping clinical features. The former, an inherited gastrointestinal malignancy predisposition, is caused by mutations in SMAD4 or BMPR1A, and the latter is a vascular malformation disorder caused by mutations in ENG or ACVRL1. All four genes encode proteins involved in the transforming-growth-factor-signaling pathway. Although there are reports of patients and families with phenotypes of both disorders combined, the genetic etiology of this association is unknown.
      Defects in SMAD4 may be a cause of colorectal cancer (CRC) [MIM:114500].
    • 序列相似性Belongs to the dwarfin/SMAD family.
      Contains 1 MH1 (MAD homology 1) domain.
      Contains 1 MH2 (MAD homology 2) domain.
    • 结构域The MH1 domain is required for DNA binding.
      The MH2 domain is required for both homomeric and heteromeric interactions and for transcriptional regulation. Sufficient for nuclear import.
    • 翻译后修饰Monoubiquitinated on Lys-519 by E3 ubiquitin-protein ligase TRIM33. Monoubiquitination hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. Deubiqitination by USP9X restores its competence to mediate TGF-beta signaling.
    • 细胞定位Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R-SMAD.
    • Information by UniProt
    • 数据库链接
    • 别名
      • (Small) Mothers Against Decapentaplegic antibody
      • Deleted in Pancreatic Carcinoma 4 antibody
      • Deleted in Pancreatic Carcinoma antibody
      • Deleted in pancreatic carcinoma locus 4 antibody
      • Deletion target in pancreatic carcinoma 4 antibody
      • DPC 4 antibody
      • DPC4 antibody
      • hSMAD4 antibody
      • JIP antibody
      • MAD homolog 4 antibody
      • MAD mothers against decapentaplegic Drosophila homolog 4 antibody
      • MAD mothers against decapentaplegic homolog 4 antibody
      • MADH 4 antibody
      • MADH4 antibody
      • Med antibody
      • Medea antibody
      • Mothers against decapentaplegic homolog 4 antibody
      • Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody
      • Mothers against DPP homolog 4 antibody
      • MYHRS antibody
      • OTTHUMP00000163548 antibody
      • SMA- and MAD-related protein 4 antibody
      • SMAD 4 antibody
      • SMAD family member 4 antibody
      • SMAD mothers against DPP homolog 4 antibody
      • SMAD4 antibody
      • SMAD4_HUMAN antibody
      see all

    Anti-Smad4 antibody 图像

    • All lanes : Anti-Smad4 antibody (ab110175) at 1/1000 dilution

      Lane 1 : SW480 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : Human skin tissue lysate
      Lane 5 : Human lung tissue lysate
      Lane 6 : Human artery tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 60 kDa
      Observed band size : 60 kDa


      Exposure time : 3 minutes

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Smad4 antibody (ab110175) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
      Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 6 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
      Lane 7 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 60 kDa
      Observed band size : 67 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 4 minutes
      The predicted molecular weight of Smad4 is 60 kDa (SwissProt), however we expect to observe a banding pattern around 70 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    Anti-Smad4 antibody (ab110175)参考文献

    ab110175 has not yet been referenced specifically in any publications.

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