Anti-Smad2 + Smad3 (phospho T8)抗体(ab63399)

概述

  • 产品名称
    Anti-Smad2 + Smad3 (phospho T8)抗体
    参阅全部 Smad2 + Smad3 一抗
  • 描述
    兔多克隆抗体to Smad2 + Smad3 (phospho T8)
  • 特异性
    This antibody detects endogenous levels of Smad2 + Smad3 only when phosphorylated at threonine 8.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthesized phosphopeptide derived from human Smad2+Smad3 around the phosphorylation site of threonine 8 (PFTPPP)

  • 阳性对照
    • Extracts from RAW264.7 cells

性能

应用

Our Abpromise guarantee covers the use of ab63399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa.
ELISA 1/20000.

靶标

  • 相关性
    SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.
  • 细胞定位
    Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
  • 数据库链接
  • 别名
    • hMAD 2 antibody
    • hMAD 3 antibody
    • hSMAD2 antibody
    • hSMAD3 antibody
    • Mad related protein 2 antibody
    • MADH2 antibody
    • MADH3 antibody
    • MADR2 antibody
    • Mothers against DPP homolog 2 antibody
    • Mothers against DPP homolog 3 antibody
    • Sma and Mad related protein 2 antibody
    • SMA and MAD related protein 3 antibody
    • SMAD 2 antibody
    • SMAD 3 antibody
    • SMAD family member 2 antibody
    • SMAD family member 3 antibody
    • smad2+3 antibody
    • smad2/3 antibody
    see all

图片

  • All lanes : Anti-Smad2 + Smad3 (phospho T8) antibody (ab63399) at 1/500 dilution

    Lane 1 : Extracts from RAW264.7 cells
    Lane 2 : Extracts from RAW264.7 cells plus phospho peptide


    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide is 5-10 ug.

实验方案

文献

This product has been referenced in:
  • Chen H  et al. Shenqiwan Ameliorates Renal Fibrosis in Rats by Inhibiting TGF-ß1/Smads Signaling Pathway. Evid Based Complement Alternat Med 2017:7187038 (2017). WB ; Rat . Read more (PubMed: 28638433) »
  • Ko JW  et al. Pine bark extract (Pycnogenol®) suppresses cigarette smoke-induced fibrotic response via transforming growth factor-ß1/Smad family member 2/3 signaling. Lab Anim Res 33:76-83 (2017). WB ; Mouse . Read more (PubMed: 28747971) »

See all 10 Publications for this product

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