概述

  • 产品名称
    Anti-Smad2 + Smad3抗体
    参阅全部 Smad2 + Smad3 一抗
  • 描述
    兔多克隆抗体to Smad2 + Smad3
  • 特异性
    ab63672 detects endogenous levels of total Smad2 and Smad3.
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic non-phosphopeptide derived from human Smad2 and Smad3 around the phosphorylation site of threonine 8 (P-F-TP-P-P).

  • 阳性对照
    • COS7 cells.

性能

应用

Our Abpromise guarantee covers the use of ab63672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa.

靶标

  • 相关性
    SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.
  • 细胞定位
    Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
  • 数据库链接
  • 别名
    • hMAD 2 antibody
    • hMAD 3 antibody
    • hSMAD2 antibody
    • hSMAD3 antibody
    • Mad related protein 2 antibody
    • MADH2 antibody
    • MADH3 antibody
    • MADR2 antibody
    • Mothers against DPP homolog 2 antibody
    • Mothers against DPP homolog 3 antibody
    • Sma and Mad related protein 2 antibody
    • SMA and MAD related protein 3 antibody
    • SMAD 2 antibody
    • SMAD 3 antibody
    • SMAD family member 2 antibody
    • SMAD family member 3 antibody
    • smad2+3 antibody
    • smad2/3 antibody
    see all

图片

  • All lanes : Anti-Smad2 + Smad3 antibody (ab63672) at 1/500 dilution

    Lane 1 : COS7 cell extracts treated with UV (15mins)
    Lane 2 : COS7 cell extracts untreated
    Lane 3 : Jurkat cell extracts treated with EGF (200ng/ml, 30mins)
    Lane 4 : Jurkat cell extracts treated with EGF (200ng/ml, 30mins) with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

实验方案

文献

This product has been referenced in:
  • Chen H  et al. Shenqiwan Ameliorates Renal Fibrosis in Rats by Inhibiting TGF-ß1/Smads Signaling Pathway. Evid Based Complement Alternat Med 2017:7187038 (2017). WB ; Rat . Read more (PubMed: 28638433) »
  • Wei MG  et al. JiaWeiDangGui Decoction Ameliorates Proteinuria and Kidney Injury in Adriamycin-Induced Rat by Blockade of TGF-ß/Smad Signaling. Evid Based Complement Alternat Med 2016:5031890 (2016). WB ; Rat . Read more (PubMed: 27403197) »

See all 4 Publications for this product

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