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Synthetic peptide corresponding to Human Smad2.
(Peptide available as
Our Abpromise guarantee covers the use of ab53100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/300 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling Smad2 (phospho S467) with ab53100. The image on the right is blocked with a phosphopeptide.
This image is courtesy of an anonymous AbreviewBlocking Step: 5% Milk for 1 hour at 18°C
This image is courtesy of an anonymous Abreview.