概述

  • 产品名称Anti-Smad2抗体[5G7]
    参阅全部 Smad2 一抗
  • 描述
    小鼠单克隆抗体[5G7] to Smad2
  • 经测试应用适用于: WB, ELISA, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Purified recombinant fragment of Human Smad2 expressed in E. Coli.

  • 阳性对照
    • Recombinant protein; Smad2-hIgGFc transfected HEK293 cell lysate; Human liver cancer and cerebellum tissues; U251 and NIH3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab119907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/200 - 1/1000.

靶标

  • 功能Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma.
  • 组织特异性Expressed at high levels in skeletal muscle, heart and placenta.
  • 序列相似性Belongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • 翻译后修饰Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
    In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.
    Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta.
  • 细胞定位Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Drosophila, homolog of, MADR2 antibody
    • hMAD-2 antibody
    • HsMAD2 antibody
    • JV18 antibody
    • JV18-1 antibody
    • JV181 antibody
    • MAD antibody
    • MAD homolog 2 antibody
    • MAD Related Protein 2 antibody
    • Mad-related protein 2 antibody
    • MADH2 antibody
    • MADR2 antibody
    • MGC22139 antibody
    • MGC34440 antibody
    • Mother against DPP homolog 2 antibody
    • Mothers against decapentaplegic homolog 2 antibody
    • Mothers against decapentaplegic, Drosophila, homolog of, 2 antibody
    • Mothers against DPP homolog 2 antibody
    • OTTHUMP00000163489 antibody
    • Sma and Mad related protein 2 antibody
    • Sma- and Mad-related protein 2 MAD antibody
    • SMAD 2 antibody
    • SMAD family member 2 antibody
    • SMAD, mothers against DPP homolog 2 antibody
    • SMAD2 antibody
    • SMAD2_HUMAN antibody
    see all

Anti-Smad2 antibody [5G7] 图像

  • Anti-Smad2 antibody [5G7] (ab119907) at 1/500 dilution + Recombinant Human Smad2 protein

    Predicted band size : 52 kDa
  • All lanes : Anti-Smad2 antibody [5G7] (ab119907) at 1/500 dilution

    Lane 1 : Non-transfected HEK293 cell lysate.
    Lane 2 : Smad2-hIgGFc transfected HEK293 cell lysate.


    Predicted band size : 52 kDa
  • ab119907 at 1/200 dilution staining Smad2 in paraffin-embedded Human liver cancer tissues by Immunohistochemistry followed by DAB staining.
  • ab119907 at 1/200 dilution staining Smad2 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry followed by DAB staining.
  • ab119907 at 1/200 dilution staining Smad2 in U251 cells by Immunofluorescence (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA showing use of ab119907.
  • ab119907 at 1/200 dilution staining Smad2 in NIH3T3 cells by Flow Cytometry (blue) and negative control (red).

Anti-Smad2 antibody [5G7] (ab119907)参考文献

This product has been referenced in:
  • Fang L  et al. Jumonji AT-rich interactive domain 1B overexpression is associated with the development and progression of glioma. Int J Mol Med 38:172-82 (2016). WB ; Human . Read more (PubMed: 27246838) »

See 1 Publication for this product

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