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Synthetic peptide: VKGWGAEYHR QDVTSTPCWI EIHLHGPLQW LDKVLTQMGS PHNPISSV, corresponding to C terminal amino acids 417-466 of Human Smad1
Our Abpromise guarantee covers the use of ab66737 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 0.625 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|