Anti-Smad1 (phospho S465)抗体(ab97689)
Key features and details
- Rabbit polyclonal to Smad1 (phospho S465)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Smad1 (phospho S465)抗体
参阅全部 Smad1 一抗 -
描述
兔多克隆抗体to Smad1 (phospho S465) -
宿主
Rabbit -
特异性
ab97689 detects endogenous levels of Smad1 only when phosphorylated at serine 465. The region surrounding Smad1 phospho S465 is highly conserved in several other Smad proteins, including Smad 5 and Smad 9. Cross reactivity with other Smad proteins is likely. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Smad1 (phospho S465).
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阳性对照
- HeLa cell extracts treated with PMA.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab97689 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97689于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa). |
靶标
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功能
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. -
组织特异性
Ubiquitous. Highest expression seen in the heart and skeletal muscle. -
序列相似性
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
翻译后修饰
Phosphorylated on serine by BMP type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. -
细胞定位
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4086 Human
- Omim: 601595 Human
- SwissProt: Q15797 Human
- Unigene: 604588 Human
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别名
- BSP-1 antibody
- BSP1 antibody
- HsMAD1 antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab97689 被引用在 3 文献中.
- Onuma TA et al. A chordate species lacking Nodal utilizes calcium oscillation and Bmp for left-right patterning. Proc Natl Acad Sci U S A 117:4188-4198 (2020). PubMed: 32029598
- Zhang F et al. Anti-osteoporosis activity of Sanguinarine in preosteoblast MC3T3-E1 cells and an ovariectomized rat model. J Cell Physiol 233:4626-4633 (2018). PubMed: 28926099
- Waki K et al. Genetic pathways for differentiation of the peripheral nervous system in ascidians. Nat Commun 6:8719 (2015). PubMed: 26515371