概述

  • 产品名称Anti-Smad1抗体
    参阅全部 Smad1 一抗
  • 描述
    兔多克隆抗体to Smad1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Sheep, Horse, Chicken, Cow, Human, Pig, Xenopus laevis, Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Smad1.

    (Peptide available as ab66722.)

  • 阳性对照
    • This antibody gave a positive signal in Mouse Heart Lysate

性能

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应用

Our Abpromise guarantee covers the use of ab46688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).

靶标

  • 功能Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
  • 组织特异性Ubiquitous. Highest expression seen in the heart and skeletal muscle.
  • 序列相似性Belongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • 翻译后修饰Phosphorylated on serine by BMP type 1 receptor kinase.
    Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
  • 细胞定位Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BSP-1 antibody
    • BSP1 antibody
    • HsMAD1 antibody
    • JV4-1 antibody
    • JV41 antibody
    • MAD homolog 1 antibody
    • MAD mothers against decapentaplegic homolog 1 antibody
    • Mad related protein 1 antibody
    • Mad-related protein 1 antibody
    • MADH1 antibody
    • MADR1 antibody
    • Mothers against decapentaplegic homolog 1 antibody
    • Mothers against DPP homolog 1 antibody
    • SMA- AND MAD-RELATED PROTEIN 1 antibody
    • SMAD 1 antibody
    • SMAD family member 1 antibody
    • SMAD mothers against DPP homolog 1 antibody
    • Smad1 antibody
    • SMAD1_HUMAN antibody
    • TGF beta signaling protein 1 antibody
    • Transforming growth factor-beta-signaling protein 1 antibody
    see all

Anti-Smad1 antibody 图像

  • Anti-Smad1 antibody (ab46688) at 1 µg/ml + Heart (Mouse) Tissue Lysate (ab27255) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • Anti-Smad1 antibody (ab46688) at 1 µg/ml + Recombinant Human Smad1 protein (ab84653) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

Anti-Smad1 antibody (ab46688)参考文献

This product has been referenced in:
  • Lu H  et al. Hepcidin promotes osteogenic differentiation through the bone morphogenetic protein 2/small mothers against decapentaplegic and mitogen-activated protein kinase/P38 signaling pathways in mesenchymal stem cells. Mol Med Rep 11:143-50 (2015). WB ; Rat . Read more (PubMed: 25351366) »
  • Das A  et al. Delivery of bioactive lipids from composite microgel-microsphere injectable scaffolds enhances stem cell recruitment and skeletal repair. PLoS One 9:e101276 (2014). WB ; Rat . Read more (PubMed: 25077607) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (HEK293 Ms Heart Ms Skeletal Muscle)
Loading amount 20 µg
Specification HEK293 Ms Heart Ms Skeletal Muscle
Gel Running Conditions Reduced Denaturing (Pierce precise protein gel 8-16%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
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Mr. Antibody Solutions

Verified customer

提交于 May 25 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"