Anti-Smad1抗体[913C1b] (ab53745)

概述

  • 产品名称
    Anti-Smad1抗体[913C1b]
    参阅全部 Smad1 一抗
  • 描述
    小鼠单克隆抗体[913C1b] to Smad1
  • 经测试应用
    适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant Smad1

  • 阳性对照
    • WB: HEK293, F2408 and NIH3T3 whole cell lysates, FC: HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab53745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
  • 组织特异性
    Ubiquitous. Highest expression seen in the heart and skeletal muscle.
  • 序列相似性
    Belongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • 翻译后修饰
    Phosphorylated on serine by BMP type 1 receptor kinase.
    Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
  • 细胞定位
    Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BSP-1 antibody
    • BSP1 antibody
    • HsMAD1 antibody
    • JV4-1 antibody
    • JV41 antibody
    • MAD homolog 1 antibody
    • MAD mothers against decapentaplegic homolog 1 antibody
    • Mad related protein 1 antibody
    • Mad-related protein 1 antibody
    • MADH1 antibody
    • MADR1 antibody
    • Mothers against decapentaplegic homolog 1 antibody
    • Mothers against DPP homolog 1 antibody
    • SMA- AND MAD-RELATED PROTEIN 1 antibody
    • SMAD 1 antibody
    • SMAD family member 1 antibody
    • SMAD mothers against DPP homolog 1 antibody
    • Smad1 antibody
    • SMAD1_HUMAN antibody
    • TGF beta signaling protein 1 antibody
    • Transforming growth factor-beta-signaling protein 1 antibody
    see all

图片

  • Using 0.5µg Anti-SMAD1 antibody (ab53745) and 0.25µg Goat anti-Mouse secondary.
  • Anti-Smad1 antibody [913C1b] (ab53745) at 1/50 dilution + HEK293 whole cell lysate at 25 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Predicted band size : 52 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Anti-Smad1 antibody [913C1b] (ab53745) at 1/50 dilution + NIH3T3 whole cell lysate at 25 µg

    Secondary
    Mouse IgG at 1/2500 dilution

    Predicted band size : 52 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
  • Anti-Smad1 antibody [913C1b] (ab53745) at 1/50 dilution + F2408 whole cell lysate at 25 µg

    Secondary
    Mouse IgG at 1/2500 dilution

    Predicted band size : 52 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)

文献

ab53745 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Fibroblasts)
Loading amount
40 µg
Specification
Fibroblasts
Gel Running Conditions
Reduced Denaturing (10% Bis-Tris with MOPS buffer)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Lukas Rambousek

Verified customer

提交于 Dec 10 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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