Anti-Smad Interacting Protein 1抗体(ab113655)

概述

  • 产品名称
    Anti-Smad Interacting Protein 1抗体
    参阅全部 Smad Interacting Protein 1 一抗
  • 描述
    山羊多克隆抗体to Smad Interacting Protein 1
  • 特异性
    This antibody is expected to recognize both reported isoforms (NP_055610.1; NP_001165124.1).
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Dog, Xenopus laevis, Zebrafish, Cynomolgus monkey, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    TDSRRQISNIKKEK

    with a Cysteine residue linker, corresponding to internal sequence amino acids 545-558 of Human Smad Interacting Protein 1 (according to NP_055610.1; NP_001165124.1).

  • 阳性对照
    • Human Liver lysate

性能

应用

Our Abpromise guarantee covers the use of ab113655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.2 - 0.5 µg/ml. Detects a band of approximately 38, 100 kDa (predicted molecular weight: 136 kDa). Primary incubation time: 1 hour.
ELISA 1/32000.

靶标

  • 功能
    Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.
  • 疾病相关
    Defects in ZEB2 are the cause of Mowat-Wilson syndrome (MWIS) [MIM:235730]; also known as Hirschsprung disease-mental retardation syndrome. A complex developmental disorder characterized by mental retardation, delayed motor development, epilepsy, microcephaly and a wide spectrum of clinically heterogeneous features suggestive of neurocristopathies at the cephalic, cardiac, and vagal levels. Some patients manifest Hirschsprung disease. Affected patients show an easily recognizable facial appearance with deep set eyes and hypertelorism, medially divergent, broad eyebrows, prominent columella, pointed chin and uplifted, notched ear lobes.
  • 序列相似性
    Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
    Contains 7 C2H2-type zinc fingers.
    Contains 1 homeobox DNA-binding domain.
  • 翻译后修饰
    Sumoylation on Lys-391 and Lys-866 is promoted by the E3 SUMO-protein ligase CBX4, and impairs interaction with CTBP1 and transcription repression activity.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ42816 antibody
    • HSPC082 antibody
    • KIAA0569 antibody
    • SIP 1 antibody
    • SIP1 antibody
    • Smad-interacting protein 1 antibody
    • SMADIP 1 antibody
    • SMADIP1 antibody
    • ZEB 2 antibody
    • Zeb2 antibody
    • ZEB2_HUMAN antibody
    • Zfhx1b antibody
    • ZFHX1B protein antibody
    • Zfx1b antibody
    • Zinc finger E box binding protein 2 antibody
    • Zinc finger E-box-binding homeobox 2 antibody
    • Zinc finger homeobox 1b antibody
    • zinc finger homeobox protein 1 antibody
    • Zinc finger homeobox protein 1b antibody
    see all

图片

  • Anti-Smad Interacting Protein 1 antibody (ab113655) at 0.2 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg

    Predicted band size : 136 kDa
    Observed band size : 38,100 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab113655 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (gel 8%)
Sample
Human Cell lysate - whole cell (ovarian cancer cell line SKOV3)
Specification
ovarian cancer cell line SKOV3
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 27°C
Username

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提交于 Aug 20 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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