Anti-SMAD1/3/5 (phospho S423 + S425)抗体(ab51451)
Key features and details
- Rabbit polyclonal to SMAD1/3/5 (phospho S423 + S425)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-SMAD1/3/5 (phospho S423 + S425)抗体 -
描述
兔多克隆抗体to SMAD1/3/5 (phospho S423 + S425) -
宿主
Rabbit -
特异性
ab51451 is specific for the phosphorylated form of the protein although minimal reactivity with nonphosphorylated human Smad3 can be observed by ELISA and WB. ab51451 also reacts with phosphorylated Smad1 and Smad5 but negligible reactivity is seen against other phosphorylated Smad family members. -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Pig, Xenopus laevis, Zebrafish -
免疫原
Synthetic peptide corresponding to Human SMAD1/3/5 aa 400-500 (phospho S423 + S425).
Database link: P84022 -
阳性对照
- WB: HEK293, HeLa, MCF-7, Jurkat, A431, A549, LNCap, MOLT-4, Ramos, Raji, A-172, NIH/3T3 Lysate; MEF lysate; Hep3B lysat.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.33% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab51451 was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51451于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (6) |
Use at an assay dependent concentration.
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WB | (3) |
1/2000 - 1/20000. Predicted molecular weight: 48 kDa. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested.
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说明 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/2000 - 1/20000. Predicted molecular weight: 48 kDa. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested. |
靶标
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数据库链接
- SwissProt: P84022 Human
- SwissProt: Q15797 Human
- SwissProt: Q99717 Human
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别名
- BSP-1 antibody
- BSP1 antibody
- DKFZP586N0721 antibody
see all
图片
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All lanes : Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451) at 1 µg/ml
Lane 1 : HEK293 lysate
Lane 2 : HeLa Lysate
Lane 3 : MCF-7 Lysate
Lane 4 : Jurkat Lysate
Lane 5 : A431 Lysate
Lane 6 : A549 Lysate
Lane 7 : LNCap Lysate
Lane 8 : MOLT-4 Lysate
Lane 9 : Ramos Lysate
Lane 10 : Raji Lysate
Lane 11 : A-172 Lysate
Lane 12 : NIH/3T3 Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 & 4-12 : Peroxidase rabbit secondary antibody at 1/30000 dilution
Lane 3 : Peroxidase rabbit secondary antibody
Predicted band size: 48 kDa -
ab51451 at 2.5 µg/ml staining epidermal keratinocytes at 40X. This image shows strong nuclear staining with localization of the antibody as the precipated red siganl and a hematoxylin purple nuclear counterstain.
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All lanes : Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451) at 1/2000 dilution
Lane 1 : MEF lysate (control)
Lane 2 : MEF lysate, after stimulation with 2 ng/ml of TGF-ß for 1 hour
Lane 3 : MEF-/-Smad3 lysate (control)
Lane 4 : MEF-/-Smad3 lysate, after stimulation with 2 ng/ml of TGF-ß for one hour
Lane 5 : Hep3B lysate (control)
Lane 6 : Hep3B lysate, after stimulation with 2 ng/ml of TGF-ß for one hour
Lane 7 : HEK 293 lysate (control)
Predicted band size: 48 kDa
Image courtesy of Ying Zhang, NIH, CCR, Bethesda, MD.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (52)
ab51451 被引用在 52 文献中.
- Baik JE et al. TGF-β1 mediates pathologic changes of secondary lymphedema by promoting fibrosis and inflammation. Clin Transl Med 12:e758 (2022). PubMed: 35652284
- Walewska E et al. The Interaction Between Nodal, Hypoxia-Inducible Factor 1 Alpha, and Thrombospondin 1 Promotes Luteolysis in Equine Corpus Luteum. Front Endocrinol (Lausanne) 10:667 (2019). PubMed: 31632347
- Ursoli Ferreira F et al. Endothelial Cells Tissue-Specific Origins Affects Their Responsiveness to TGF-ß2 during Endothelial-to-Mesenchymal Transition. Int J Mol Sci 20:N/A (2019). PubMed: 30678183
- Riemondy KA et al. Single cell RNA sequencing identifies TGFß as a key regenerative cue following LPS-induced lung injury. JCI Insight 5:N/A (2019). PubMed: 30913038
- Chung YH et al. BMP-2 restoration aids in recovery from liver fibrosis by attenuating TGF-ß1 signaling. Lab Invest N/A:N/A (2018). WB ; Mouse . PubMed: 29789683