重组
RabMAb

Anti-SMAD 1 + SMAD9抗体[EPR2290(2)] (ab108965)

概述

  • 产品名称
    Anti-SMAD 1 + SMAD9抗体[EPR2290(2)]
  • 描述
    兔单克隆抗体[EPR2290(2)] to SMAD 1 + SMAD9
  • 经测试应用
    适用于: WBmore details
    不适用于: Flow Cyt,ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human SMAD 1 + SMAD9.

  • 阳性对照
    • 293T, A673, Human heart and HeLa lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab108965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa).
  • 应用说明
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • 靶标

    • 功能
      Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
    • 组织特异性
      Ubiquitous. Highest expression seen in the heart and skeletal muscle.
    • 序列相似性
      Belongs to the dwarfin/SMAD family.
      Contains 1 MH1 (MAD homology 1) domain.
      Contains 1 MH2 (MAD homology 2) domain.
    • 翻译后修饰
      Phosphorylated on serine by BMP type 1 receptor kinase.
      Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
    • 细胞定位
      Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BSP-1 antibody
      • BSP1 antibody
      • HsMAD1 antibody
      • JV4-1 antibody
      • MAD homolog 1 antibody
      • Mad-related protein 1 antibody
      • MADH1 antibody
      • MADR1 antibody
      • Mothers against decapentaplegic homolog 1 antibody
      • Mothers against DPP homolog 1 antibody
      • SMAD 1 antibody
      • SMAD family member 1 antibody
      • Smad1 antibody
      • SMAD1_HUMAN antibody
      • Transforming growth factor-beta-signaling protein 1 antibody
      see all

    图片

    • All lanes : Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] (ab108965) at 1/1000 dilution

      Lane 1 : 293T lysate
      Lane 2 : A673 lysate
      Lane 3 : Human heart lysate
      Lane 4 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lanes 1, 3 & 4 : HRP labelled goat anti-rabbit at 1/2000 dilution


      Predicted band size : 52 kDa
      Observed band size : 58 kDa (why is the actual band size different from the predicted?)

    实验方案

    文献

    ab108965 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    We carry products which recognize mouse SMAD1/9, SMAD2/3 or SMAD1/5/9. There is no antibody currently on our catalog which recognizes all forms of SMAD in mouse. I have added links to our anti- SMAD1/9, SMAD2/3 and SMAD1/5/9 antibodies for your review ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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