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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Smac/ Diablo (C terminal). The exact sequence is proprietary.
This product is a recombinant rabbit monoclonal antibody.
Alternative versions available:
Anti-Smac / Diablo antibody (Alexa Fluor® 488) [Y12] (ab199317)
Anti-Smac / Diablo antibody (Alexa Fluor® 647) [Y12] (ab199503)
Anti-Smac / Diablo antibody (HRP) [Y12] (ab199505)
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab32023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 27 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|IP||1/10 - 1/80.|
|Flow Cyt||1/10 - 1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Smac / Diablo with ab32023 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI.
Immunofluorescence analysis of IMR32 cells, staining Smac / Diablo with ab32023. Cells were fixed with 4% paraformaldehyde before incubating with primary antibody. An Alexa Fluor® conjugated anti-rabbit IgG was used as the secondary antibody.