概述
- 产品名称Anti-Smac / Diablo抗体[SMAC 17 1-87]
参阅全部 Smac / Diablo 一抗 - 描述小鼠单克隆抗体[SMAC 17 1-87] to Smac / Diablo
- 经测试应用适用于: IP, ICC/IF, WBmore details
- 种属反应性与反应: Rat, Human
- 免疫原
Recombinant full length Human Smac/ Diablo with a His tag at C terminal end.
- 阳性对照
- Human tissues.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- 存储溶液Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... - 纯度Protein G purified
- 克隆单克隆
- 克隆编号SMAC 17 1-87
- 同种型IgG1
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab111893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| ICC/IF | 1/20 - 1/200. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa). |
靶标
- 功能Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).
- 组织特异性Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes.
- 结构域The mature N-terminus mediates interaction with XIAP.
- 细胞定位Mitochondrion. Released into the cytosol when cells undergo apoptosis.
- Information by UniProt
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数据库链接
- Entrez Gene: 56616 Human
- Entrez Gene: 288753 Rat
- Omim: 605219 Human
- SwissProt: Q9NR28 Human
- Unigene: 169611 Human
- Unigene: 9090 Rat
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别名
- 0610041G12Rik antibody
- DBLOH_HUMAN antibody
- DBOH antibody
see all
Anti-Smac / Diablo antibody [SMAC 17 1-87] 图像
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![Immunocytochemistry/ Immunofluorescence - Anti-Smac / Diablo antibody [SMAC 17 1-87] (ab111893)](http://www.abcam.cn/ps/products/111/ab111893/Images/ab111893-201437-ab111893iccA431.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Smac / Diablo antibody [SMAC 17 1-87] (ab111893)ab111893 labelling Smac / Diablo (green) in the cytoplasm of A431 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse IgG was used as the secondary antibody. Red (phalloidiin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
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![Immunocytochemistry/ Immunofluorescence - Anti-Smac / Diablo antibody [SMAC 17 1-87] (ab111893)](http://www.abcam.cn/ps/products/111/ab111893/Images/ab111893-201436-ab111893iccMCF7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Smac / Diablo antibody [SMAC 17 1-87] (ab111893)ab111893 labelling Smac / Diablo (green) in the cytoplasm of MCF-7 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse IgG was used as the secondary antibody. Red (phalloidiin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
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![Immunocytochemistry/ Immunofluorescence - Anti-Smac / Diablo antibody [SMAC 17 1-87] (ab111893)](http://www.abcam.cn/ps/products/111/ab111893/Images/ab111893-201433-ab111893iccPC12.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Smac / Diablo antibody [SMAC 17 1-87] (ab111893)ab111893 labelling Smac / Diablo (green) in the cytoplasm of PC12 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse IgG was used as the secondary antibody. Red (phalloidiin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
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![Western blot - Anti-Smac / Diablo antibody [SMAC 17 1-87] (ab111893)](http://www.abcam.cn/ps/products/111/ab111893/Images/ab111893-201432-ab111893wb.jpg)
Western blot - Anti-Smac / Diablo antibody [SMAC 17 1-87] (ab111893)All lanes : Anti-Smac / Diablo antibody [SMAC 17 1-87] (ab111893) at 1/1000 dilution
Lane 1 : 293 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : PC12 cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size : 27 kDa
Observed band size : 21 kDa (why is the actual band size different from the predicted?)
Anti-Smac / Diablo antibody [SMAC 17 1-87] (ab111893)参考文献
ab111893 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"