概述

  • 产品名称Anti-Smac / Diablo抗体
    参阅全部 Smac / Diablo 一抗
  • 描述
    兔多克隆抗体to Smac / Diablo
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Peptide (

    SDEGADQEEEAYLRED

    )corresponding to amino acids 222 to 237 of murineSmac/DIABLO (2).

  • 阳性对照
    • Mouse heart tissue lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab8115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Can be blocked with Smac / Diablo peptide (ab8381).
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).
  • 组织特异性Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes.
  • 结构域The mature N-terminus mediates interaction with XIAP.
  • 细胞定位Mitochondrion. Released into the cytosol when cells undergo apoptosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 0610041G12Rik antibody
    • DBLOH_HUMAN antibody
    • DBOH antibody
    • DFNA64 antibody
    • diablo antibody
    • Diablo homolog (Drosophila) antibody
    • Diablo homolog antibody
    • Diablo homolog mitochondrial antibody
    • Diablo IAP binding mitochondrial protein antibody
    • Diablo like protein antibody
    • DIABLO S antibody
    • Direct IAP binding protein with low pI antibody
    • Direct IAP-binding protein with low pI antibody
    • FLJ10537 antibody
    • FLJ25049 antibody
    • mitochondrial antibody
    • Mitochondrial Smac protein antibody
    • Second mitochondria derived activator of caspase antibody
    • Second mitochondria-derived activator of caspase antibody
    • SMAC 3 antibody
    • Smac antibody
    • Smac protein antibody
    • SMAC3 antibody
    see all

Anti-Smac / Diablo antibody 图像

  • All lanes : Anti-Smac / Diablo antibody (ab8115) at 1 µg/ml

    Lane 1 : Mouse heart tissue lysate with absence of blocking peptide
    Lane 2 : Mouse heart tissue lysate with presence of blocking peptide
    Lane 3 : Rat heart tissue lysate with absence of blocking peptide


    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  • ab8115 at 2µg/ml staining of Smac/Diablo in mouse spleen tissue by IHC

  • Immunofluorescence of Smac in Mouse Spleen cells using ab8115 at 10 ug/ml.

Anti-Smac / Diablo antibody (ab8115)参考文献

This product has been referenced in:
  • Shephard F  et al. A mitochondrial location for haemoglobins--dynamic distribution in ageing and Parkinson's disease. Mitochondrion 14:64-72 (2014). WB ; Mouse . Read more (PubMed: 24333691) »
  • Heazell AE  et al. Altered expression of regulators of caspase activity within trophoblast of normal pregnancies and pregnancies complicated by preeclampsia. Reprod Sci 15:1034-43 (2008). WB ; Human . Read more (PubMed: 19088373) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 100mM sodium cytrate
Permeabilization No
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Ms. Hadas Pahima

Verified customer

提交于 Oct 11 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"