Anti-Smac / Diablo抗体(ab23450)

概述

  • 产品名称Anti-Smac / Diablo抗体
    参阅全部 Smac / Diablo 一抗
  • 描述
    兔多克隆抗体to Smac / Diablo
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Rabbit, Human
    预测可用于: Cow, Dog, Pig
  • 免疫原

    A region within synthetic peptide: LQVEEVHQLS RKAETKLAEA QIEELRQKTQ EEGEERAESE QEAYLRED, corresponding to C terminal amino acids 139-186 of Human Smac/ Diablo

  • 阳性对照
    • HepG2 cell lysate for WB. Paraffin embedded human kidney tissue for IHC.

性能

应用

Our Abpromise guarantee covers the use of ab23450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/15000.
WB Use a concentration of 1 - 1.25 µg/ml. Predicted molecular weight: 27 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).
  • 组织特异性Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes.
  • 结构域The mature N-terminus mediates interaction with XIAP.
  • 细胞定位Mitochondrion. Released into the cytosol when cells undergo apoptosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 0610041G12Rik antibody
    • DBLOH_HUMAN antibody
    • DBOH antibody
    • DFNA64 antibody
    • diablo antibody
    • Diablo homolog (Drosophila) antibody
    • Diablo homolog antibody
    • Diablo homolog mitochondrial antibody
    • Diablo IAP binding mitochondrial protein antibody
    • Diablo like protein antibody
    • DIABLO S antibody
    • Direct IAP binding protein with low pI antibody
    • Direct IAP-binding protein with low pI antibody
    • FLJ10537 antibody
    • FLJ25049 antibody
    • mitochondrial antibody
    • Mitochondrial Smac protein antibody
    • Second mitochondria derived activator of caspase antibody
    • Second mitochondria-derived activator of caspase antibody
    • SMAC 3 antibody
    • Smac antibody
    • Smac protein antibody
    • SMAC3 antibody
    see all

Anti-Smac / Diablo antibody 图像

  • Anti-Smac / Diablo antibody (ab23450) at 1 µg/ml + HepG2 lysate

    Secondary
    HRP conjugated anti-Rabbit IgG (1: 50,000-100,000)

    Predicted band size : 27 kDa
    Observed band size : 14 kDa (why is the actual band size different from the predicted?)
  • ab23450 at 4-8 ug/ml staining human Smac in kidney cells by immunohistochemistry (Paraffin embedded tissue). Epithelial cells of renal tubule (indicated with arrows). Magnification 400x.

Anti-Smac / Diablo antibody (ab23450)参考文献

ab23450 has not yet been referenced specifically in any publications.

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