Anti-Smac / Diablo抗体[8H5AA3] (ab110288)


  • 产品名称Anti-Smac / Diablo抗体[8H5AA3]
    参阅全部 Smac / Diablo 一抗
  • 描述
    小鼠单克隆抗体[8H5AA3] to Smac / Diablo
  • 经测试应用适用于: ICC, Flow Cyt, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant Human SMAC, TIM22 protein

  • 阳性对照
    • Human HDFn cells and HeLa cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal testis.
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.



Our Abpromise guarantee covers the use of ab110288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 5 µg/ml.


  • 功能Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).
  • 组织特异性Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes.
  • 结构域The mature N-terminus mediates interaction with XIAP.
  • 细胞定位Mitochondrion. Released into the cytosol when cells undergo apoptosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 0610041G12Rik antibody
    • DBLOH_HUMAN antibody
    • DBOH antibody
    • DFNA64 antibody
    • diablo antibody
    • Diablo homolog (Drosophila) antibody
    • Diablo homolog antibody
    • Diablo homolog mitochondrial antibody
    • Diablo IAP binding mitochondrial protein antibody
    • Diablo like protein antibody
    • DIABLO S antibody
    • Direct IAP binding protein with low pI antibody
    • Direct IAP-binding protein with low pI antibody
    • FLJ10537 antibody
    • FLJ25049 antibody
    • mitochondrial antibody
    • Mitochondrial Smac protein antibody
    • Second mitochondria derived activator of caspase antibody
    • Second mitochondria-derived activator of caspase antibody
    • SMAC 3 antibody
    • Smac antibody
    • Smac protein antibody
    • SMAC3 antibody
    see all

Anti-Smac / Diablo antibody [8H5AA3] 图像

  • HeLa cells were stained with 1 µg/mL Smac / Diablo antibody (ab110288) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • Immunocytochemistry image of Smac / Diablo stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (ab110288) at 1µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
  • IHC image of Smac / Diablo staining in Human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab110288, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Smac / Diablo antibody [8H5AA3] (ab110288)参考文献

ab110288 has not yet been referenced specifically in any publications.

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