Anti-Smac/Diablo抗体[8H5AA3] (ab110288)
Key features and details
- Mouse monoclonal [8H5AA3] to Smac/Diablo
- Suitable for: ICC, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-Smac/Diablo抗体[8H5AA3]
参阅全部 Smac/Diablo 一抗 -
描述
小鼠单克隆抗体[8H5AA3] to Smac/Diablo -
宿主
Mouse -
经测试应用
适用于: ICC, Flow Cyt, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein. This information is considered to be commercially sensitive.
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阳性对照
- Human HDFn cells and HeLa cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal testis.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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纯度
Ammonium Sulphate Precipitation -
纯化说明
This antibody has homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
克隆
单克隆 -
克隆编号
8H5AA3 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110288于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
Use a concentration of 1 µg/ml.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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IHC-P |
Use a concentration of 5 µg/ml.
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说明 |
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ICC
Use a concentration of 1 µg/ml. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
IHC-P
Use a concentration of 5 µg/ml. |
靶标
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功能
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). -
组织特异性
Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. -
结构域
The mature N-terminus mediates interaction with XIAP. -
细胞定位
Mitochondrion. Released into the cytosol when cells undergo apoptosis. - Information by UniProt
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数据库链接
- Entrez Gene: 56616 Human
- Omim: 605219 Human
- SwissProt: Q9NR28 Human
- Unigene: 169611 Human
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别名
- 0610041G12Rik antibody
- DBLOH_HUMAN antibody
- DBOH antibody
see all
图片
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HeLa cells were stained with 1 µg/mL Smac / Diablo antibody (ab110288) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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Immunocytochemistry image of Smac / Diablo stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (ab110288) at 1µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
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IHC image of Smac / Diablo staining in Human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab110288, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab110288 被引用在 1 文献中.
- Wang C et al. Bone morphogenetic protein-2 exhibits therapeutic benefits for osteonecrosis of the femoral head through induction of cartilage and bone cells. Exp Ther Med 15:4298-4308 (2018). PubMed: 29849774