概述

  • 产品名称Anti-SM22 alpha抗体
    参阅全部 SM22 alpha 一抗
  • 描述
    小鼠单克隆抗体to SM22 alpha
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length Human SM22 alpha protein (AAH04927) with tag.

  • 阳性对照
    • SM22 alpha transfected 293T cell lysate. Recombinant tagged Human SM22 alpha protein.

性能

应用

Our Abpromise guarantee covers the use of ab77442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
ELISA Use at an assay dependent dilution.

靶标

  • 功能Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
  • 序列相似性Belongs to the calponin family.
    Contains 1 calponin-like repeat.
    Contains 1 CH (calponin-homology) domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 22 kDa actin binding protein antibody
    • 22 kDa actin-binding protein antibody
    • Human 22kDa smooth muscle protein antibody
    • Protein WS3-10 antibody
    • SM22 alpha antibody
    • SM22 antibody
    • SM22-alpha antibody
    • SMCC antibody
    • Smooth muscle protein 22-alpha antibody
    • TAGL_HUMAN antibody
    • TAGLN antibody
    • TAGLN1 antibody
    • Transgelin antibody
    • Transgelin variant 2 antibody
    • WS3 10 antibody
    see all

Anti-SM22 alpha antibody 图像

  • All lanes : Anti-SM22 alpha antibody (ab77442) at 1 µg/ml

    Lane 1 : SM22 alpha transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 23 kDa
    Observed band size : 23 kDa
  • Anti-SM22 alpha antibody (ab77442) at 1 µg/ml + Recombinant tagged Human SM22 alpha protein at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 23 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

Anti-SM22 alpha antibody (ab77442)参考文献

ab77442 has not yet been referenced specifically in any publications.

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