Anti-TAGLN/Transgelin抗体(ab155272)
Key features and details
- Rabbit polyclonal to TAGLN/Transgelin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-TAGLN/Transgelin抗体
参阅全部 TAGLN/Transgelin 一抗 -
描述
兔多克隆抗体to TAGLN/Transgelin -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Chicken, Cow -
免疫原
Recombinant fragment corresponding to Human TAGLN/Transgelin (internal sequence).
Database link: Q01995 -
阳性对照
- Recombinant Human SM22 alpha protein (ab101469) can be used as a positive control in WB. HepG2 whole cell lysate, Mouse liver whole cell lysate, A-10 cells treated with MG132. HeLa cells. Ca922 xenograft.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab155272于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
|
说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence. -
序列相似性
Belongs to the calponin family.
Contains 1 calponin-like repeat.
Contains 1 CH (calponin-homology) domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 6876 Human
- Entrez Gene: 21345 Mouse
- Entrez Gene: 25123 Rat
- SwissProt: Q01995 Human
- SwissProt: P37804 Mouse
- SwissProt: P31232 Rat
- Unigene: 410977 Human
- Unigene: 283283 Mouse
see all -
别名
- 22 kDa actin-binding protein antibody
- Protein WS3-10 antibody
- SM22 antibody
see all
图片
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Anti-TAGLN/Transgelin antibody (ab155272) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 23 kDa
12% SDS PAGE -
Anti-TAGLN/Transgelin antibody (ab155272) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 23 kDa
12% SDS PAGE -
All lanes : Anti-TAGLN/Transgelin antibody (ab155272) at 1/5000 dilution
Lane 1 : A-10 whole cell lysate
Lane 2 : A-10 whole cell lysate treated with MG132
Lysates/proteins at 35 µg per lane.
Predicted band size: 23 kDa -
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling SM22 alpha with ab155272 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, labeling SM22 alpha with ab155272 at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab155272 被引用在 13 文献中.
- Yang J et al. Single-cell profiling reveals molecular basis of malignant phenotypes and tumor microenvironments in small bowel adenocarcinomas. Cell Discov 8:92 (2022). PubMed: 36104333
- Wang G et al. Curcumin-mediated Photodynamic Therapy Inhibits the Phenotypic Transformation, Migration, and Foaming of Oxidized Low-density Lipoprotein-treated Vascular Smooth Muscle Cells by Promoting Autophagy. J Cardiovasc Pharmacol 78:308-318 (2021). PubMed: 34091481
- Mohabeer AL et al. Deletion of type VIII collagen reduces blood pressure, increases carotid artery functional distensibility and promotes elastin deposition. Matrix Biol Plus 12:100085 (2021). PubMed: 34693248
- Cao X et al. MSC-derived exosomal lncRNA SNHG7 suppresses endothelial-mesenchymal transition and tube formation in diabetic retinopathy via miR-34a-5p/XBP1 axis. Life Sci 272:119232 (2021). PubMed: 33600866
- Jiang L et al. Smad3 signalling affects high glucose-induced podocyte injury via regulation of the cytoskeletal protein transgelin. Nephrology (Carlton) 25:659-666 (2020). PubMed: 32034833