概述

  • 产品名称
  • 描述
    兔多克隆抗体to SLX4
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 900 - 1000 of Human SLX4.

    (Peptide available as ab116498.)

  • 阳性对照
    • This antibody gave a positive signal in HEK293 and U2OS whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab100997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 200 kDa).

靶标

  • 功能
    Regulatory subunit that interacts with and increases the activity of different structure-specific endonucleases. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures originating from replication and recombination intermediates and from DNA damage. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating Holliday junctions (HJs). Resolves HJs by generating two pairs of ligatable, nicked duplex products. Interacts with the structure-specific ERCC4-ERCC1 endonuclease and promotes the cleavage of bubble structures. Interacts with the structure-specific MUS81-EME1 endonuclease and promotes the cleavage of 3'-flap and replication fork-like structures. SLX4 is required for recovery from alkylation-induced DNA damage and is involved in the resolution of DNA double-strand breaks.
  • 序列相似性
    Belongs to the SLX4 family.
    Contains 1 BTB (POZ) domain.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus. Localizes to sites of DNA dammage.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BTB (POZ) domain containing 12 antibody
    • BTB/POZ domain-containing protein 12 antibody
    • BTBD12 antibody
    • FANCP antibody
    • KIAA1784 antibody
    • KIAA1987 antibody
    • MUS312 antibody
    • SLX 4 antibody
    • slx4 antibody
    • SLX4 structure-specific endonuclease subunit homolog (S. cerevisiae) antibody
    • SLX4_HUMAN antibody
    • Structure-specific endonuclease subunit slx4 antibody
    see all

图片

  • All lanes : Anti-SLX4 antibody (ab100997) at 1 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 200 kDa
    Observed band size: 200 kDa
    Additional bands at: 175 kDa (possible isoform), 42 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 minutes


    This antibody was raised against an immunogen that is predicted to recognize isoforms 1 and 2 of SLX4. The predicted molecular weights of isoforms 1 and 2 are 200 kDa and 166 kDa respectively.

实验方案

文献

ab100997 has not yet been referenced specifically in any publications.

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