概述

  • 产品名称Anti-SLURP1抗体
    参阅全部 SLURP1 一抗
  • 描述
    兔多克隆抗体to SLURP1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide within Human SLURP1 aa 50 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P55000

  • 阳性对照
    • This antibody gave a positive signal in WB within Human Skin tissue lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal skin.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab93840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 11 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性SLURP1 (Secreted LY6/PLAUR domain containing 1) belongs to the Ly6/uPAR family but lacks a GPI-anchoring signal sequence. SLURP1 potentiates alpha-7 nicotinic acetylcholine receptors (CHRNA7) in keratinocytes and is implicated in maintaining the physiological and structural integrity of the keratinocyte layers of the skin. It is a marker of late differentiation of the skin and has an antitumor activity. Mutations in the SLURP1 gene are the cause of Mal de Meleda (MDM), a rare autosomal recessive genetic disease, characterized by inflammatory palmoplantar keratoderma.
  • 细胞定位Secreted
  • 数据库链接
  • 别名
    • Anti neoplastic urinary protein antibody
    • ANUP antibody
    • ARS antibody
    • ARS component B antibody
    • ARS(component B) 81/S antibody
    • ArsB antibody
    • LY6LS antibody
    • Lymphocyte antigen 6 like secreted antibody
    • MDM antibody
    • Results in cobblestone changes in the skin of the palm antibody
    • Secreted Ly 6/uPAR related protein 1 antibody
    • Secreted LY6/PLAUR domain containing 1 antibody
    • Secreted Ly6/uPAR related protein 1 antibody
    • SLURP 1 antibody
    see all

Anti-SLURP1 antibody 图像

  • IHC image of SLURP1 staining in Human normal skin formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93840, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Anti-SLURP1 antibody (ab93840) at 1 µg/ml + Human skin tissue lysate - total protein (ab30166) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 11 kDa
    Observed band size : 9 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa (possible non-specific binding),58 kDa (possible non-specific binding),95 kDa (possible non-specific binding).

    Exposure time : 8 minutes

    The band observed at 9 kDa could potentially be a cleaved form of SLURP1 due to the presence of a 22 amino acid signal peptide.

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab93840 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

Anti-SLURP1 antibody (ab93840)参考文献

ab93840 has not yet been referenced specifically in any publications.

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