Anti-SLUG抗体[mAbcam51772] (ab51772)

概述

  • 产品名称Anti-SLUG抗体[mAbcam51772]
    参阅全部 SLUG 一抗
  • 描述
    小鼠单克隆抗体[mAbcam51772] to SLUG
  • 经测试应用适用于: Flow Cyt, WB, ICC/IF, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chicken, Cow, Pig, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human SLUG.

    (Peptide available as ab27840.)

  • 阳性对照
    • Mouse Skin Tissue Lysate
  • 常规说明

    Alternative versions available:
    Anti-SLUG antibody (FITC) [mAbcam51772] (ab150214)

性能

应用

Our Abpromise guarantee covers the use of ab51772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. PubMed: 24932263ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 30 kDa.
ICC/IF 1/100.
IHC-Fr 1/100.

靶标

  • 功能Transcriptional repressor. Involved in the generation and migration of neural crest cells.
  • 组织特异性Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.
  • 疾病相关Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) [MIM:608890]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
  • 序列相似性Belongs to the snail C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC10182 antibody
    • Neural crest transcription factor Slug antibody
    • Protein snail homolog 2 antibody
    • Slug (chicken homolog) zinc finger protein antibody
    • Slug homolog zinc finger protein antibody
    • Slug zinc finger protein antibody
    • SLUGH antibody
    • SLUGH 1 antibody
    • SLUGH1 antibody
    • SNAI 2 antibody
    • Snai2 antibody
    • SNAI2_HUMAN antibody
    • Snail 2 antibody
    • Snail homolog 2 antibody
    • Snail2 antibody
    • WS 2D antibody
    • WS2D antibody
    • Zinc finger protein SLUG antibody
    • Zinc finger protein SNAI2 antibody
    see all

Anti-SLUG antibody [mAbcam51772] 图像

  • All lanes : Anti-SLUG antibody [mAbcam51772] (ab51772)

    Lane 1 : Untransfected MCF7 cells
    Lane 2 : MCF7 cells transfected with SLUG
    Lane 3 : MCF7 cells transfected with Snail


    Predicted band size : 30 kDa
    Observed band size : 30 kDa
  • Anti-SLUG antibody [mAbcam51772] (ab51772) at 5 µg/ml + Mouse skin tissue lysate - total protein (ab4025) at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
    Additional bands at : 34 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes
  • All lanes : Anti-SLUG antibody [mAbcam51772] (ab51772) at 1 µg/ml

    Lane 1 : Skin tissue lysate from Slug heterozygous mouse
    Lane 2 : Skin tissue lysate from Slug knockout mouse


    Performed under reducing conditions.

    Predicted band size : 30 kDa
  • ab51772 staining SLUG in mouse mammary gland cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with PF4% Triton 0,5% for 5min and blocking with 10% serum for 1 hour at RT was performed. Samples were incubated with primary antibody (1/100) for 1 hour. An Alexa Fluor®488-conjugated goat polyclonal to mouse IgG (H+L) was used at dilution at 1/750 as secondary antibody. Blue colour in the image represents DAPI staining.

    See Abreview

Anti-SLUG antibody [mAbcam51772] (ab51772)参考文献

This product has been referenced in:
  • Kudinov AE  et al. Musashi-2 (MSI2) supports TGF-ß signaling and inhibits claudins to promote non-small cell lung cancer (NSCLC) metastasis. Proc Natl Acad Sci U S A 113:6955-60 (2016). WB . Read more (PubMed: 27274057) »
  • Sun Y  et al. Slug overexpression induces stemness and promotes hepatocellular carcinoma cell invasion and metastasis. Oncol Lett 7:1936-1940 (2014). WB, Flow Cyt ; Human . Read more (PubMed: 24932263) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (Human prostate cancer cell)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 30 µg
Specification Human prostate cancer cell
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 31 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample Human Cell (endothelial cell)
Specification endothelial cell
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 29 2014

Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Jan 23 2013

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La secuencia de aminoácidos del inmunógeno de ab5177...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Apteronotus leptorhynchus Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Dr. Ruxandra Sirbulescu

Verified customer

提交于 Nov 14 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Ms. Mayssaa NASSOUR

Verified customer

提交于 Jul 27 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse mammary gland)
Specification mouse mammary gland
Fixative Paraformaldehyde
Permeabilization Yes - PF4% triton 0,5% 5min
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Ms. Mayssaa NASSOUR

Verified customer

提交于 Jul 22 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (skin, comma D ß cell line)
Loading amount 40 µg
Specification skin, comma D ß cell line
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Mayssaa NASSOUR

Verified customer

提交于 Jul 21 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa 293T)
Loading amount 30 µg
Specification HeLa 293T
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Sep 03 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"