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Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human SLUG.
(Peptide available as ab27840.)
Our Abpromise guarantee covers the use of ab51772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 24932263ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 30 kDa.|
ab51772 staining SLUG in mouse mammary gland cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with PF4% Triton 0,5% for 5min and blocking with 10% serum for 1 hour at RT was performed. Samples were incubated with primary antibody (1/100) for 1 hour. An Alexa Fluor®488-conjugated goat polyclonal to mouse IgG (H+L) was used at dilution at 1/750 as secondary antibody. Blue colour in the image represents DAPI staining.