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Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human SLUG.
Our Abpromise guarantee covers the use of ab27568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 30-34 kDa (predicted molecular weight: 30 kDa).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 18716062|
Secondary antibody - goat anti-rabbit HRP (preadsorbed)
Image courtesy of Human Protein Atlas
ab27568 staining in human placenta, showing staining of the trophoblastic cells (in brown). Paraffin embedded placental tissue was incubated with ab27568 (1:750 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab27568 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
This image is courtesy of Archana Dhasarathy, Ph.D., NCab27568 detects a band of 34 kDa in MCF7 cells that have been transfected with a vector that overexpresses the SLUG protein. This antibody does not crossreact with the SNAIL protein, as it did not recognize this protein in MCF7 cells overexpressing SNAIL. ab27568 was unable to detect endogenous SLUG in MDA-MB231 cells (not shown).
ab27568 staining mouse embryonic stem cells by ICC/IF. Cells were formaldehyde fixed and permeabilized in 0.1% Triton X-100 prior to blocking with 0.1% BSA and 1% serum for 30 minutes at 21°C. The primary antibody was diluted 1/100 and incubated for 2 hours at 21°C. An Alexa Fluor® 488 conjugated goat anti-rabbit antibody, diluted 1/500, was used as the secondary.