Anti-SLUG抗体(ab106077)

概述

  • 产品名称Anti-SLUG抗体
    参阅全部 SLUG 一抗
  • 描述
    兔多克隆抗体to SLUG
  • 经测试应用适用于: IHC-Fr, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human SLUG.

  • 阳性对照
    • This antibody gave a positive signal in MCF7 overexpressing SLUG whole cell lysate.
  • 常规说明This antibody has only been tested on overexpressing cell lysates; we have no data on the detection of the endogenous protein.

性能

应用

Our Abpromise guarantee covers the use of ab106077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 30 kDa).

靶标

  • 功能Transcriptional repressor. Involved in the generation and migration of neural crest cells.
  • 组织特异性Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.
  • 疾病相关Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) [MIM:608890]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
  • 序列相似性Belongs to the snail C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC10182 antibody
    • Neural crest transcription factor Slug antibody
    • Protein snail homolog 2 antibody
    • Slug (chicken homolog) zinc finger protein antibody
    • Slug homolog zinc finger protein antibody
    • Slug zinc finger protein antibody
    • SLUGH antibody
    • SLUGH 1 antibody
    • SLUGH1 antibody
    • SNAI 2 antibody
    • Snai2 antibody
    • SNAI2_HUMAN antibody
    • Snail 2 antibody
    • Snail homolog 2 antibody
    • Snail2 antibody
    • WS 2D antibody
    • WS2D antibody
    • Zinc finger protein SLUG antibody
    • Zinc finger protein SNAI2 antibody
    see all

Anti-SLUG antibody 图像

  • All lanes : Anti-SLUG antibody (ab106077) at 1 µg/ml

    Lane 1 : MCF7 cell lysate overexpressing SLUG protein
    Lane 2 : MCF7 cell lysate overexpressing SNAIL protein
    Lane 3 : MCF7 cell lysate transfected with vector control plasmid
    Lane 4 : Untransfected MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes
  • ab106077 staining SLUG on an E9.5 mouse embryo section by immunohistochemistry. The tissue was paraformaldehyde fixed and permeabilized in PBST prior to blocking in NOVOCASTRA protein block for 30 minutes at 25°C. The primary antibody was diluted 1/25 and incubated with the sample for 48 hour at 4°C. An Alexa Fluor 555 conjugated donkey anti-rabbit antibody, diluted 1/1000, was used as the secondary. Nuclear staining shown with DAPI.

    See Abreview

  • All lanes : Anti-SLUG antibody (ab106077) at 1/2000 dilution

    Lane 1 : MDA-MB-231 whole cell lysate
    Lane 2 : MDA-MB-231 whole cell lysate - sh Slug 1
    Lane 3 : MDA-MB-231 whole cell lysate - sh Slug 2
    Lane 4 : MDA-MB-231 whole cell lysate - sh Snail 1

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 35,65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa (possible non-specific binding).

    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    Blocked with 5% milk for 1 hour at 22°C.

    Primary incubation at 4°C for 18 hours.

    See Abreview

Anti-SLUG antibody (ab106077)参考文献

ab106077 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-12% NuPage)
Sample Human Cell lysate - whole cell (MDA-MB-231 cells)
Specification MDA-MB-231 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Jan 21 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample Human Cell (HMLE-Slug over-expressed cells)
Specification HMLE-Slug over-expressed cells
Permeabilization Yes - 0.02% Triton X-100
Fixative Paraformaldehyde
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提交于 Jun 19 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E9.5 mouse embryo section)
Specification E9.5 mouse embryo section
Fixative Paraformaldehyde
Permeabilization Yes - PBST (PBS / 0.5% v/v TritonX100 (Sigma))
Blocking step NOVOCASTRA protein block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
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提交于 Oct 10 2012

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Application Western blot
Sample Human Cell lysate - whole cell (MCF7 breast cancer)
Loading amount 10 µg
Specification MCF7 breast cancer
Treatment 3 of the samples treated with Snail, Slug or Control adenovirus for 2 days
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jun 18 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"