Anti-SLTM抗体(ab69481)

概述

  • 产品名称Anti-SLTM抗体
    参阅全部 SLTM 一抗
  • 描述
    小鼠多克隆抗体to SLTM
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human SLTM protein (ENSP00000369883, 1 a.a. ~ 478 a.a)

  • 阳性对照
    • Human colon tissue lysate. SLTM transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab69481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 135 kDa (predicted molecular weight: 117 kDa).

靶标

  • 相关性When SLTM is overexpressed it acts as a general inhibitor of transcription that eventually leads to apoptosis.
  • 细胞定位Nuclear. Note: Detected in punctate structures.
  • 数据库链接
  • 别名
    • DKFZp762G052 antibody
    • FLJ10005 antibody
    • FLJ13213 antibody
    • MET antibody
    • Modulator of estrogen induced transcription antibody
    • SAFB like transcription modulator antibody
    see all

Anti-SLTM antibody 图像

  • Anti-SLTM antibody (ab69481) at 1/500 dilution + Human colon lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 117 kDa
    Observed band size : 135 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-SLTM antibody (ab69481) at 1/500 dilution

    Lane 1 : SLTM transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 117 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

Anti-SLTM antibody (ab69481)参考文献

ab69481 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (TS-Rs26)
Gel Running Conditions Reduced Denaturing (8% gel)
Loading amount 60 µg
Specification TS-Rs26
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Verified customer

提交于 Jan 14 2016

Thank you for your reply.

I have issued a replacement vial of ab69481 and it should be dispatched today. Apologies for any inconvenience.

Do not hesitate to contact us should you have further questions.

Thank you for contacting us.

I am sorry that the vial you received did not contain the full amount of the product. You are correct it should contain ˜50µl of antibody.

It is important to centrifuge any vial containing liquid...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"