概述

  • 产品名称Anti-SLPI抗体
    参阅全部 SLPI 一抗
  • 描述
    兔多克隆抗体to SLPI
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human SLPI.

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Human Lung; Human Spleen; Human Small Intestine; Human Colon. It also gave a positive signal in HeLa, Hek293 and HepG2 cells in IF.

性能

应用

Our Abpromise guarantee covers the use of ab82337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/250. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).

靶标

  • 功能Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. May prevent elastase-mediated damage to oral and possibly other mucosal tissues.
  • 组织特异性Mucous fluids.
  • 序列相似性Contains 2 WAP domains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ALK 1 antibody
    • ALK1 antibody
    • ALP antibody
    • Antileukoprotease antibody
    • Antileukoproteinase 1 antibody
    • Antileukoproteinase antibody
    • Antileukoproteinase1 antibody
    • BLPI antibody
    • Human seminal protease inhibitor antibody
    • HUSI 1 antibody
    • HUSI antibody
    • HUSI I antibody
    • HUSI-1 antibody
    • MPI antibody
    • Mucus proteinase inhibitor antibody
    • Protease inhibitor WAP4 antibody
    • Secretory leukocyte peptidase inhibitor antibody
    • Secretory leukocyte protease inhibitor antibody
    • Seminal proteinase inhibitor antibody
    • SLPI antibody
    • SLPI_HUMAN antibody
    • WAP four disulfide core domain protein 4 antibody
    • WAP four-disulfide core domain protein 4 antibody
    • WAP4 antibody
    • WFDC4 antibody
    see all

Anti-SLPI antibody 图像

  • All lanes : Anti-SLPI antibody (ab82337) at 1/250 dilution

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)
    Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 4 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
    Additional bands at : 29 kDa,48 kDa,8 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab82337 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82337, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) Hek293 and HepG2 cells at 5µg/ml.

Anti-SLPI antibody (ab82337)参考文献

ab82337 has not yet been referenced specifically in any publications.

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