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Purified human antileukoprotease (ALP).
Our Abpromise guarantee covers the use of ab17157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50. Detects a band of approximately 12 kDa (predicted molecular weight: 14 kDa). If bands larger than 12-14 kDa are seen then this can be explained by aggregation of SLPI. Due to cationic character of SLPI it is also possible that SLPI gives a band at 14 kDa instead of 12 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
Immunohistochemical analysis on human tonsil frozen sections with ab17157 at a 1:50 dilution.
ab17157 staining SLPI in Human lung cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with the HOPE technique and blocked for 15 minutes. Samples were incubated with primary antibody (1/4000) for 1 hour at 25°C. An undiluted HRP-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.
ab17157 has not yet been referenced specifically in any publications.
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