Reacts with Synechocystis sp.
Possibly crossreacts with Crocosphaera watsonii due to sequence homology.
, corresponding to amino acids 271-286 of Synechocystis sp. PCC 6803 sll0528.
- Synechocystis sp. PCC 6803 membranes.
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Preservative: 0.01% Sodium Azide
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in the following tested applications.
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||1/2000. Detects a band of approximately 48 kDa (predicted molecular weight: 40 kDa).
sll0528, a transmembrane metallo protease from Synechocystis sp. PCC 6803, belongs to the SP2-type metallopeptidases family, the key players of intramembrane proteolysis.
Cell membrane; Multi pass membrane protein
- Putative zinc metalloprotease sll0528 antibody
Western blot - sll0528 antibody (ab65550)
Anti-sll0528 antibody (ab65550) at 1/2000 dilution + Synechocystis sp. PCC 6803
membranes at 3 µgSecondary
anti-rabbit at 1/30000 dilution
Developed using the ECL techniquePredicted band size :
40 kDaObserved band size :
48 kDa (why is the actual band size different from the predicted?
)Additional bands at :
18 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
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