Anti-SLK抗体(ab70231)

概述

  • 产品名称Anti-SLK抗体
    参阅全部 SLK 一抗
  • 描述
    兔多克隆抗体to SLK
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between C terminal residues 1200 and 1235 of Human SLK (NP_055535.2)

  • 阳性对照
    • Whole cell lysate from MCF7, HeLa or 293T cells.

性能

应用

Our Abpromise guarantee covers the use of ab70231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 143 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 相关性SLK is a serine/threonine protein kinase that is involved in cytoskeletal reorganization and apoptosis. SLK is a microtubule-associated protein inducing actin stress fiber disassembly, it also mediates apoptosis and it is ubiquitously expressed; highest expression is found in heart and in skeletal muscle. There are two named isoforms.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • CTCL tumor antigen se20 9 antibody
    • LOSK antibody
    • se20 9 antibody
    • Serine/threonine protein kinase 2 antibody
    • SLK antibody
    • SNF1 sucrose nonfermenting like kinase antibody
    • STE20 like serine/threonine-protein kinase antibody
    • STE20 related kinase antibody
    • STK2 antibody
    see all

Anti-SLK antibody 图像

  • All lanes : Anti-SLK antibody (ab70231) at 1 µg/ml

    Lane 1 : MCF whole cell lysate at 50 µg
    Lane 2 : MCF whole cell lysate at 15 µg
    Lane 3 : MCF whole cell lysate at 5 µg
    Lane 4 : HeLa whole cell lysate at 15 µg
    Lane 5 : 293T whole cell lysate at 15 µg


    Predicted band size : 143 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)
  • MCF7 whole cell lysate (1 mg/IP; 1/4 of IP loaded) probed with ab70231 at 1µg/ml, subsequent to IP with ab70231 at 3µg/mg lysate (lane 1) or with rabbit IgG control (lane 2)
    Detection: chemiluminescence with exposure time of 10 seconds.

Anti-SLK antibody (ab70231)参考文献

ab70231 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"