概述

  • 产品名称
  • 描述
    兔多克隆抗体to SLK
  • 经测试应用
    适用于: ELISA, ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide from the C terminus of human SLK.

  • 阳性对照
    • Extracts from COLO205 cells, human skeletal muscle tissue, HepG2 cells.

性能

应用

Our Abpromise guarantee covers the use of ab65113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 143 kDa (predicted molecular weight: 143 kDa).
IHC-P 1/50 - 1/100.

靶标

  • 相关性
    SLK is a serine/threonine protein kinase that is involved in cytoskeletal reorganization and apoptosis. SLK is a microtubule-associated protein inducing actin stress fiber disassembly, it also mediates apoptosis and it is ubiquitously expressed; highest expression is found in heart and in skeletal muscle. There are two named isoforms.
  • 细胞定位
    Cytoplasmic
  • 数据库链接
  • 别名
    • CTCL tumor antigen se20 9 antibody
    • LOSK antibody
    • se20 9 antibody
    • Serine/threonine protein kinase 2 antibody
    • SLK antibody
    • SNF1 sucrose nonfermenting like kinase antibody
    • STE20 like serine/threonine-protein kinase antibody
    • STE20 related kinase antibody
    • STK2 antibody
    see all

Anti-SLK antibody 图像

  • This image shows human skeletal muscle tissue stained with ab65113 at 1/50 - 1/100 dilution; with and without the immunising peptide.
  • All lanes : Anti-SLK antibody (ab65113) at 1/500 dilution

    Lane 1 : extracts from COLO205 cells
    Lane 2 : extracts from COLO205 cells with immunising peptide


    Predicted band size : 143 kDa
    Observed band size : 143 kDa
  • Immunofluorescence analysis of HepG2 cells, using ab65113 antibody at 1/500-1/1000 dilution

Anti-SLK antibody (ab65113)参考文献

This product has been referenced in:
  • Tranchevent LC  et al. Identification of protein features encoded by alternative exons using Exon Ontology. Genome Res 27:1087-1097 (2017). ICC/IF ; Human . Read more (PubMed: 28420690) »
  • Müller T  et al. ASAP1 promotes tumor cell motility and invasiveness, stimulates metastasis formation in vivo, and correlates with poor survival in colorectal cancer patients. Oncogene : (2010). WB ; Human . Read more (PubMed: 20154719) »

See all 2 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela Cells)
Loading amount
20 µg
Specification
Hela Cells
Gel Running Conditions
Non-reduced Denaturing (7.5% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

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提交于 Jun 14 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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