概述

  • 产品名称Anti-Slit2抗体
    参阅全部 Slit2 一抗
  • 描述
    兔多克隆抗体to Slit2
  • 经测试应用适用于: ICC/IF, WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Human
    预测可用于: Xenopus laevis
  • 免疫原

    Synthetic peptide:

    YFIPGTEDYRSKLSGDC

    conjugated to KLH, corresponding to amino acids 484-500 of Human SLIT2.

  • 阳性对照
    • Chicken spinal cord whole cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab7665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/2000. Detects a band of approximately 165 kDa (predicted molecular weight: 170 kDa). Incubate primary antibody blocking buffer (5% goat serum + 0.5% non-fat dry milk in PBS) other blocking buffers cause a poor signal.
IHC-Fr Use at an assay dependent concentration. PubMed: 20498081

靶标

  • 功能Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.
  • 组织特异性Fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea and other tissues examined.
  • 序列相似性Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 7 EGF-like domains.
    Contains 1 laminin G-like domain.
    Contains 20 LRR (leucine-rich) repeats.
    Contains 4 LRRCT domains.
    Contains 4 LRRNT domains.
  • 结构域The leucine-rich repeat domain is sufficient for guiding both axon projection and neuronal migration, in vitro.
  • 细胞定位Secreted. The C-terminal cleavage protein is more diffusible than the larger N-terminal protein that is more tightly cell associated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Drad 1 antibody
    • E030015M03Rik antibody
    • E130320P19Rik antibody
    • FLJ14420 antibody
    • OTTHUMP00000158695 antibody
    • OTTHUMP00000217852 antibody
    • OTTHUMP00000217853 antibody
    • OTTHUMP00000217854 antibody
    • Slil 3 antibody
    • Slil3 antibody
    • Slit 2 antibody
    • Slit homolog 2 (Drosophila) antibody
    • Slit homolog 2 antibody
    • Slit homolog 2 protein antibody
    • Slit homolog 2 protein C-product antibody
    • Slit-2 antibody
    • Slit2 antibody
    • SLIT2_HUMAN antibody
    see all

Anti-Slit2 antibody 图像

  • Anti-Slit2 antibody (ab7665) at 1/1350 dilution + Chicken spinal cord whole cell lysate at 30 µg

    Secondary
    IRDye™800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

    Predicted band size : 170 kDa
    Observed band size : 165 kDa (why is the actual band size different from the predicted?)
  • ab7665 staining Slit2 in rat glioma cell line C6 by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab7665 at a 1/80 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-rabbit polyclonal, used at a 1/400 dilution. Nuclei are counterstained with DAPI.

    See Abreview

  • ab7665 staining Slit2 in human glioblastoma cell line D54MG by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab7665 at a 1/80 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-rabbit polyclonal, used at a 1/400 dilution. Nuclei are counterstained with DAPI.

    See Abreview

  • ab7665 staining Slit2 in human glioblastoma cell line by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0,1% Triton X 100 in PBS, blocked with 0.5% BSA for 30 minutes at room temperature and then incubated with ab7665 at a 1/50 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/400 dilution. Nuclei are counterstained with DAPI.

    See Abreview

Anti-Slit2 antibody (ab7665)参考文献

This product has been referenced in:
  • Pilling D  et al. Fibroblasts secrete Slit2 to inhibit fibrocyte differentiation and fibrosis. Proc Natl Acad Sci U S A 111:18291-6 (2014). Human . Read more (PubMed: 25489114) »
  • Marlow R  et al. Vascular Robo4 restricts proangiogenic VEGF signaling in breast. Proc Natl Acad Sci U S A 107:10520-5 (2010). IHC-Fr ; Mouse . Read more (PubMed: 20498081) »

See all 2 Publications for this product

Product Wall

As discussed the mouse cross reactivity was added due to its citation in PMID 20498081. We unfortunately do not have any other mouse cross reactivity data available.

I have cheked the SLIT2 antibodies from different vendoes. The observed band...

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Thank you very much for your email.

The preparation of tissue and cell lysates looks fine to me. The antibody should have worked, I however have following recommendations, I am sure these will help to improve the results.

- Heat lys...

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Thanks you very much for sending the details and image.

I have reviewed the protocol being used and also have checked the provide image; I am sorry I have few more questions to ask;

- How the cell lysates were prepared? What buffer ...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I a...

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Thank you for contacting us.

The Anti-Slit2 antibody (ab7665) should detect a band at ˜165 kDa indeed, not 55 kDa.

In order to understand the detection of a band at 55 kDa, have you performed a "no primary" control (incub...

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DISCOUNT CODE: XXXXXXXXXX
Expiration date: 2012-07-05

I am very pleased to hear you would like to accept our offer and test ab7665 in IHC-P. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this of...

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Thank you for your enquiry.

Here is a link to more information regarding our Abreview system: http://www.abcam.com/abreviews

I hope this is helpful. Please contact me again if you have any further questions.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma cell line)
Specification human glioblastoma cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X 100 in PBS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jan 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (glioblastoma cell line C6)
Specification glioblastoma cell line C6
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X 100 in PBS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jan 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma cell line D54MG)
Specification human glioblastoma cell line D54MG
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X 100
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Aug 30 2011

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