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Our Abpromise guarantee covers the use of ab7665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500 - 1/2000. Detects a band of approximately 165 kDa (predicted molecular weight: 170 kDa). Incubate primary antibody blocking buffer (5% goat serum + 0.5% non-fat dry milk in PBS) other blocking buffers cause a poor signal.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20498081|
Molecular weight estimation was made by comparison to prestained molecular weight markers