Anti-Slit1抗体(ab115892)

概述

  • 产品名称Anti-Slit1抗体
    参阅全部 Slit1 一抗
  • 描述
    小鼠多克隆抗体to Slit1
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chicken, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to Mouse Slit1 aa 497-504 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    CSAKEQYFIPGTEDYHLN

  • 阳性对照
    • IHC-P: Human Kidney tissue; Human Pancreas tissue
  • 常规说明Avoid repeated freezing and thawing.

性能

应用

Our Abpromise guarantee covers the use of ab115892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 167 kDa.
ELISA 1/10000 - 1/50000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent dilution.

靶标

  • 功能Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.
  • 组织特异性Predominantly expressed in adult forebrain. Expressed in fetal brain, lung and kidney.
  • 序列相似性Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 9 EGF-like domains.
    Contains 1 laminin G-like domain.
    Contains 20 LRR (leucine-rich) repeats.
    Contains 4 LRRCT domains.
    Contains 4 LRRNT domains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA0813 antibody
    • MEGF 4 antibody
    • MEGF4 antibody
    • MGC164811 antibody
    • Multiple EGF-like domains protein 4 antibody
    • Multiple epidermal growth factor like domains 4 antibody
    • Multiple epidermal growth factor-like domains protein 4 antibody
    • OTTHUMP00000059392 antibody
    • SLIL 1 antibody
    • SLIL1 antibody
    • Slit 1 antibody
    • SLIT 3 antibody
    • Slit homolog 1 antibody
    • Slit homolog 1 protein antibody
    • Slit-1 antibody
    • Slit1 antibody
    • SLIT1_HUMAN antibody
    • SLIT3 antibody
    see all

Anti-Slit1 antibody 图像

  • ab115892, at 5 ug/ml, staining Slit1 in formalin fixed, paraffin embedded Human kidney tissue by Immunohistochemistry. Developed using Goat anti rabbit biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab115892, at 5 ug/ml, staining Slit1 in formalin fixed, paraffin embedded Human pancreas tissue by Immunohistochemistry. Developed using Goat anti rabbit biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Anti-Slit1 antibody (ab115892)参考文献

ab115892 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris (MOPS buffer))
Sample Human Cell lysate - whole cell (HEK293T with construct expressing human Slit1-myc)
Specification HEK293T with construct expressing human Slit1-myc
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 51% · Temperature: RT°C
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提交于 Dec 12 2013

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