Anti-Slit1抗体(ab10984)

概述

  • 产品名称Anti-Slit1抗体
    参阅全部 Slit1 一抗
  • 描述
    兔多克隆抗体to Slit1
  • 特异性The smaller strong bands observed in the WB shown are likely to be cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40kDa -160kDa, (Little et al, 2002).
  • 经测试应用适用于: WBmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    CSAKEQYFIPGTEDYHLN

    conjugated to KLH, corresponding to amino acids 487-504 of Mouse Slit1.

性能

应用

Our Abpromise guarantee covers the use of ab10984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/500. Detects a band of approximately 168 kDa (predicted molecular weight: 168 kDa).

    Is unsuitable for IHC-P.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.
    • 组织特异性Predominantly expressed in adult forebrain. Expressed in fetal brain, lung and kidney.
    • 序列相似性Contains 1 CTCK (C-terminal cystine knot-like) domain.
      Contains 9 EGF-like domains.
      Contains 1 laminin G-like domain.
      Contains 20 LRR (leucine-rich) repeats.
      Contains 4 LRRCT domains.
      Contains 4 LRRNT domains.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • KIAA0813 antibody
      • MEGF 4 antibody
      • MEGF4 antibody
      • MGC164811 antibody
      • Multiple EGF-like domains protein 4 antibody
      • Multiple epidermal growth factor like domains 4 antibody
      • Multiple epidermal growth factor-like domains protein 4 antibody
      • OTTHUMP00000059392 antibody
      • SLIL 1 antibody
      • SLIL1 antibody
      • Slit 1 antibody
      • SLIT 3 antibody
      • Slit homolog 1 antibody
      • Slit homolog 1 protein antibody
      • Slit-1 antibody
      • Slit1 antibody
      • SLIT1_HUMAN antibody
      • SLIT3 antibody
      see all

    Anti-Slit1 antibody 图像



    • Predicted band size : 168 kDa

      ab10984-Rabbit polyclonal to Slit1 (1/500)

      Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000); 20µg of cell lysate per lane. Expected molecular weight: 168 kDa; Exposure time: 5 min
           
      Lane 1: Rat Brain tissue lysate
      Lane 2: Mouse Brain tissue Lysate

      Note: The smaller strong bands observed in this WB are likely to be cleavage products. A number of  cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40kDa -160kDa, (Little et al, 2002).

      ab10984-Rabbit polyclonal to Slit1 (1/500) Secondary ab: Goat anti-rabbit HRP ab6721 (1/5000); 20µg of cell lysate per lane. Expected molecular weight: 168 kDa; Exposure time: 5 min Lane 1: Rat Brain tissue lysate Lane 2: Mouse Brain tissue Lysate Note: The smaller strong bands observed in this WB are likely to be cleavage products. A number of

    Anti-Slit1 antibody (ab10984)参考文献

    ab10984 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (e16 T/S whole embryo body)
    Specification e16 T/S whole embryo body
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
    Username

    Mr. Carl Hobbs

    Verified customer

    提交于 Mar 22 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"