The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
SLIRP is a RNA binding protein that acts as a nuclear receptor corepressor. It may act by binding the SRA RNA, and repressing the SRA-mediated nuclear receptor coactivation. SLIRP binds the STR7 loop of SRA RNA. It is also able to repress glucocorticoid (GR), androgen (AR), thyroid (TR) and VDR-mediated transactivation. It contains 1 RRM (RNA recognition motif) domain. SLIRP is predominantly founs in mitochondrial with a limited role in the nucleus where it is recruited to nuclear receptor target promoters.
Mitochondrial and Nuclear; Predominantly mitochondrial.
Carpp LN et al. Quantitative proteomic analysis of host-virus interactions reveals a role for Golgi brefeldin A resistance factor 1 (GBF1) in dengue infection. Mol Cell Proteomics13:2836-54 (2014).
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Clemente P et al. hCOA3 stabilizes cytochrome c oxidase 1 (COX1) and promotes cytochrome c oxidase assembly in human mitochondria. J Biol Chem288:8321-31 (2013).
Read more (PubMed: 23362268) »