Anti-Slingshot homolog 1抗体(ab76943)

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概述

  • 产品名称
    Anti-Slingshot homolog 1抗体
    参阅全部 Slingshot homolog 1 一抗
  • 描述
    兔多克隆抗体to Slingshot homolog 1
  • 经测试应用
    适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 675 and 725 of human Slingshot homolog 1 (NP_061857.2)

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab76943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 116 kDa).
IP Use at 2-5 - 5 µg/mg of lysate.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能
    Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.
  • 序列相似性
    Belongs to the protein-tyrosine phosphatase family.
    Contains 1 tyrosine-protein phosphatase domain.
  • 翻译后修饰
    Phosphorylated. Inhibitory phosphorylation by PAK4 promotes binding to YWHAZ. Phosphorylation at Ser-978 is decreased by stimuli which promote actin reorganization and lamellipodia formation. Can be dephosphorylated and activated by PPP3CA/calcineurin A. Phosphorylation decreases immediately prior to telophase.
  • 细胞定位
    Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cleavage furrow. Midbody. Also recruited to actin rich membrane protrusions such as lamellipodia, which may allow local control of actin dynamics at sites of cell locomotion. Also localized to the cleavage furrow and the midbody during cytokinesis.
  • Information by UniProt
  • 数据库链接
    • 别名
      • AW551225 antibody
      • Coro1c antibody
      • coronin, actin binding protein 1C antibody
      • Gm1394 antibody
      • Gm1395 antibody
      • hSSH-1L antibody
      • mSSH 1L antibody
      • OTTMUSP00000031415 antibody
      • Protein phosphatase Slingshot homolog 1 antibody
      • Slingshot 1 antibody
      • slingshot 1L antibody
      • slingshot homolog 1 antibody
      • slingshot like 1 antibody
      • Slingshot1 antibody
      • SSH 1 antibody
      • SSH 1L antibody
      • SSH-1L antibody
      • SSH-like protein 1 antibody
      • Ssh1 antibody
      • SSH1_HUMAN antibody
      see all

    Anti-Slingshot homolog 1 antibody 图像

    • Western blot - Slingshot homolog 1 antibody (ab76943)
      Western blot - Slingshot homolog 1 antibody (ab76943)
      All lanes : Anti-Slingshot homolog 1 antibody (ab76943) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 116 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    • Immunoprecipitation - Slingshot homolog 1 antibody (ab76943)
      Immunoprecipitation - Slingshot homolog 1 antibody (ab76943)
      ab76943 at 1µg/ml staining Slingshot homolog 1 in HeLa whole cell lysate immunoprecipitated using ab76943 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    • Immunocytochemistry/ Immunofluorescence-Slingshot homolog 1 antibody(ab76943)
      Immunocytochemistry/ Immunofluorescence-Slingshot homolog 1 antibody(ab76943)
      ICC/IF image of ab76943 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76943, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-Slingshot homolog 1 antibody (ab76943)参考文献

    This product has been referenced in:
    • Bielig H  et al. The cofilin phosphatase slingshot homolog 1 (SSH1) links NOD1 signaling to actin remodeling. PLoS Pathog 10:e1004351 (2014). WB, IP ; Human . Read more (PubMed: 25187968) »
    • Leonard A  et al. Thrombin selectively engages LIM kinase 1 and slingshot-1L phosphatase to regulate NF-?B activation and endothelial cell inflammation. Am J Physiol Lung Cell Mol Physiol 305:L651-64 (2013). Read more (PubMed: 24039253) »

    See all 5 Publications for this product

    发表研究结果有使用 ab76943？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    1 Abreview or Q&A

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    Votes: Highest First

    I am interested in using both your anti-LIMK (cat# ab95186) and anti-SSH (cat# ab76943) antibodies for immunopercipitation. I took a look at your protocol online for IP, but it didn’t say (or I missed it) what lysis/IP buffer was used.  I normally ly...

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    Thank you for contacting Abcam. Regarding the lysis/IP buffer to use with these antibodies, they have been tested with a buffer using the following formulation: IP lysis Buffer 12.5 ml 1M NaCl (250mM) 2.5 ml 1M Tris (50mM) 500ul 0.5M EDTA (5m...

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