- 数据表
- 具体的参考文献 (8)
- 实验方案
概述
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产品名称Anti-Slingshot homolog 1抗体
参阅全部 Slingshot homolog 1 一抗 -
描述兔多克隆抗体to Slingshot homolog 1
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宿主Rabbit
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经测试应用适用于: WB, IP, ICC/IFmore details
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种属反应性与反应: Human
预测可用于: Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residues 675 and 725 of human Slingshot homolog 1 (NP_061857.2)
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阳性对照
- HeLa and 293T whole cell lysates.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明ab76943 was affinity purified using an epitope specific to Slingshot homolog 1 immobilized on solid support.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab76943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 116 kDa). | |
| IP | Use at 2-5 - 5 µg/mg of lysate. | |
| ICC/IF | Use a concentration of 1 µg/ml. |
靶标
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功能Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.
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序列相似性Belongs to the protein-tyrosine phosphatase family.
Contains 1 tyrosine-protein phosphatase domain. -
翻译后修饰Phosphorylated. Inhibitory phosphorylation by PAK4 promotes binding to YWHAZ. Phosphorylation at Ser-978 is decreased by stimuli which promote actin reorganization and lamellipodia formation. Can be dephosphorylated and activated by PPP3CA/calcineurin A. Phosphorylation decreases immediately prior to telophase.
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细胞定位Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cleavage furrow. Midbody. Also recruited to actin rich membrane protrusions such as lamellipodia, which may allow local control of actin dynamics at sites of cell locomotion. Also localized to the cleavage furrow and the midbody during cytokinesis.
- Information by UniProt
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数据库链接
- Entrez Gene: 54434 Human
- Omim: 606778 Human
- SwissProt: Q8WYL5 Human
- Unigene: 199763 Human
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别名
- AW551225 antibody
- Coro1c antibody
- coronin, actin binding protein 1C antibody
see all
图片
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Western blot - Anti-Slingshot homolog 1 antibody (ab76943)All lanes : Anti-Slingshot homolog 1 antibody (ab76943) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 116 kDa
Observed band size: 150 kDa (why is the actual band size different from the predicted?) -
Immunoprecipitation - Anti-Slingshot homolog 1 antibody (ab76943)ab76943 at 1µg/ml staining Slingshot homolog 1 in HeLa whole cell lysate immunoprecipitated using ab76943 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). -
Immunocytochemistry/ Immunofluorescence - Anti-Slingshot homolog 1 antibody (ab76943)ICC/IF image of ab76943 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76943, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
文献
This product has been referenced in:
- Maimaiti Y et al. SSH1 expression is associated with gastric cancer progression and predicts a poor prognosis. BMC Gastroenterol 18:12 (2018). Read more (PubMed: 29338701) »
- Majumder P et al. Cellular levels of Grb2 and cytoskeleton stability are correlated in a neurodegenerative scenario. Dis Model Mech 10:655-669 (2017). Read more (PubMed: 28360125) »
客户评价及客户问答
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