antigen sequence: YCRSGTSVLH MNSRQAFDFL KTKERQSKYN LINEGSPPSK IMKAVYQNIS ESNPAYEVFQ TDTIEYGEIL SFPESPSIE, corresponding to amino acids 135-213 of Human SLFN13.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-SLFN13 antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-SLFN13 antibody (ab121737)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121737 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLFN13 antibody (ab121737)
ab121737 at 1/40 dilution staining SLFN13 in Paraffin-embedded Human Pancreas tissue by Immunohistochemistry. Note: strong cytoplasmic positivity in a subset of exocrine glandular cells.
Anti-SLFN13 antibody (ab121737)参考文献
has not yet been referenced specifically in any publications.
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