antigen sequence: REVLGCAKEN VDPDSLRRKI EQAIYKLPCV HFCQPQRPIT FTLKIVDVLK RGELYGYACM IRVNPFCCAV FSEAPNSWI, corresponding to amino acids 255-333 of Human SLFN11.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent concentration. Predicted molecular weight: 103 kDa.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-SLFN11 antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-SLFN11 antibody (ab121731)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121731 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-SLFN11 antibody (ab121731)
Anti-SLFN11 antibody (ab121731) + MOLT-4Secondary
SLFN11Predicted band size :
103 kDaObserved band size :
Western blot analysis in human cell line MOLT-4 staining SLFN11 with ab121731
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLFN11 antibody (ab121731)
ab121731 at 1/15 dilution staining SLFN11 in Paraffin-embedded Human Pancreas tissue by Immunohistochemistry. Note: moderate cytoplasmic positivity in islet cells.
Anti-SLFN11 antibody (ab121731)参考文献
has not yet been referenced specifically in any publications.
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