WB, ICC/IF, IHC-Pmore details
antigen sequence: REVLGCAKEN VDPDSLRRKI EQAIYKLPCV HFCQPQRPIT FTLKIVDVLK RGELYGYACM IRVNPFCCAV FSEAPNSWI, corresponding to amino acids 255-333 of Human SLFN11.
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- 存储溶液pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
- 纯度Immunogen affinity purified
- Compatible Secondaries
- Positive Controls
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent concentration. Predicted molecular weight: 103 kDa.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
- 序列相似性Belongs to the Schlafen family.
Information by UniProt
- Schlafen family member 11 antibody
- SLFN11 antibody
- SLFN8/9 antibody
- SLN11_HUMAN antibody
Anti-SLFN11 antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-SLFN11 antibody (ab121731)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121731 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-SLFN11 antibody (ab121731)
Anti-SLFN11 antibody (ab121731) + MOLT-4
Predicted band size : 103 kDa
Observed band size : 103 kDa
Western blot analysis in human cell line MOLT-4 staining SLFN11 with ab121731
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLFN11 antibody (ab121731)
ab121731 at 1/15 dilution staining SLFN11 in Paraffin-embedded Human Pancreas tissue by Immunohistochemistry. Note: moderate cytoplasmic positivity in islet cells.
Anti-SLFN11 antibody (ab121731)参考文献
has not yet been referenced specifically in any publications.
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