Anti-SLCO1C1抗体(ab83972)

概述

  • 产品名称Anti-SLCO1C1抗体
    参阅全部 SLCO1C1 一抗
  • 描述
    兔多克隆抗体to SLCO1C1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within the internal amino acids 180-229 (VDTSSSMWIY VFLGNLLRGI GETPIQPLGI AYLDDFASED NAAFYIGCVQ) of Human SLCO1C1 (NP_059131)

  • 阳性对照
    • Jurkat cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab83972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 79 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Mediates the Na(+)-independent high affinity transport of organic anions such as the thyroid hormones thyroxine (T4) and rT3. Other potential substrates, such as triiodothyronine (T3), 17-beta-glucuronosyl estradiol, estrone-3-sulfate and sulfobromophthalein (BSP) are transported with much lower efficiency. May play a signifiant role in regulating T4 flux into and out of the brain.
  • 组织特异性Highly expressed in brain and in Leydig cells in testis. Detected in many brain regions with the exception of pons and cerebellum. Not strongly enriched in cerebral microvessels.
  • 序列相似性Belongs to the organo anion transporter (TC 2.A.60) family.
    Contains 1 Kazal-like domain.
  • 细胞定位Cell membrane. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells. Localized to the apical membrane and basal surfaces of choroid plexus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • OAT-RP-5 antibody
    • OATP-14 antibody
    • OATP-F antibody
    • OATP1 antibody
    • OATP1C1 antibody
    • OATPRP5 antibody
    • Organic anion transporter F antibody
    • Organic anion transporter polypeptide-related protein 5 antibody
    • organic anion transporting polypeptide 14 antibody
    • Organic anion-transporting polypeptide 14 antibody
    • SLC21A14 antibody
    • Slco1c1 antibody
    • SO1C1_HUMAN antibody
    • solute carrier family 21 (organic anion transporter), member 14 antibody
    • Solute carrier family 21 member 14 antibody
    • Solute carrier organic anion transporter family member 1C1 antibody
    • solute carrier organic anion transporter family, member 1C1 antibody
    • Thyroxine transporter antibody
    see all

Anti-SLCO1C1 antibody 图像

  • Anti-SLCO1C1 antibody (ab83972) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 79 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

Anti-SLCO1C1 antibody (ab83972)参考文献

ab83972 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Ovarian cancer (OVCAR-3))
Permeabilization Yes - 70% ethanol, 30 min at -20
Specification Ovarian cancer (OVCAR-3)
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Abcam user community

Verified customer

提交于 Nov 25 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Testis)
Permeabilization Yes - 0.1% Triton X-100 in Blocking Solution
Specification Testis
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative Davidson's Fixative
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Mr. Yoshiyuki Henning

Verified customer

提交于 Nov 17 2016

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (7.5% gel)
Sample Mouse Tissue lysate - whole (Liver)
Specification Liver
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Oct 09 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Inner Ear)
Specification Inner Ear
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100 when using PFA. None when using Methanol.
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jan 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"