Anti-SLCO1C1抗体(ab83972)

概述

  • 产品名称
    Anti-SLCO1C1抗体
    参阅全部 SLCO1C1 一抗
  • 描述
    兔多克隆抗体to SLCO1C1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within the internal amino acids 180-229 (VDTSSSMWIY VFLGNLLRGI GETPIQPLGI AYLDDFASED NAAFYIGCVQ) of Human SLCO1C1 (NP_059131)

  • 阳性对照
    • Jurkat cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab83972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 79 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Mediates the Na(+)-independent high affinity transport of organic anions such as the thyroid hormones thyroxine (T4) and rT3. Other potential substrates, such as triiodothyronine (T3), 17-beta-glucuronosyl estradiol, estrone-3-sulfate and sulfobromophthalein (BSP) are transported with much lower efficiency. May play a signifiant role in regulating T4 flux into and out of the brain.
  • 组织特异性
    Highly expressed in brain and in Leydig cells in testis. Detected in many brain regions with the exception of pons and cerebellum. Not strongly enriched in cerebral microvessels.
  • 序列相似性
    Belongs to the organo anion transporter (TC 2.A.60) family.
    Contains 1 Kazal-like domain.
  • 细胞定位
    Cell membrane. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells. Localized to the apical membrane and basal surfaces of choroid plexus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • OAT-RP-5 antibody
    • OATP-14 antibody
    • OATP-F antibody
    • OATP1 antibody
    • OATP1C1 antibody
    • OATPRP5 antibody
    • Organic anion transporter F antibody
    • Organic anion transporter polypeptide-related protein 5 antibody
    • organic anion transporting polypeptide 14 antibody
    • Organic anion-transporting polypeptide 14 antibody
    • SLC21A14 antibody
    • Slco1c1 antibody
    • SO1C1_HUMAN antibody
    • solute carrier family 21 (organic anion transporter), member 14 antibody
    • Solute carrier family 21 member 14 antibody
    • Solute carrier organic anion transporter family member 1C1 antibody
    • solute carrier organic anion transporter family, member 1C1 antibody
    • Thyroxine transporter antibody
    see all

图片

  • Anti-SLCO1C1 antibody (ab83972) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 79 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

ab83972 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (whole larvea)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
whole larvea
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 23 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Ovarian cancer (OVCAR-3))
Permeabilization
Yes - 70% ethanol, 30 min at -20
Specification
Ovarian cancer (OVCAR-3)
Username

Abcam user community

Verified customer

提交于 Nov 25 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis)
Permeabilization
Yes - 0.1% Triton X-100 in Blocking Solution
Specification
Testis
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Davidson's Fixative
Username

Mr. Yoshiyuki Henning

Verified customer

提交于 Nov 17 2016

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (7.5% gel)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 09 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Inner Ear)
Specification
Inner Ear
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 when using PFA. None when using Methanol.
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jan 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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