概述

  • 产品名称Anti-SLC9A8抗体
    参阅全部 SLC9A8 一抗
  • 描述
    兔多克隆抗体to SLC9A8
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide from within internal sequence amino acids 531-580: AKYLNPFFTR RLTQEDLHHG RIQMKTLTNK WYEEVRQGPS GSEDDEQELL, of Human SLC9A8 (NP_056081)

  • 阳性对照
    • HepG2 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

靶标

  • 功能Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
  • 组织特异性Ubiquitous. Strongly expressed in skeletal muscle and kidney.
  • 序列相似性Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
  • 细胞定位Golgi apparatus membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Na(+)/H(+) exchanger 8 antibody
    • NHE 8 antibody
    • NHE-8 antibody
    • NHE8 antibody
    • SL9A8_HUMAN antibody
    • Slc9a8 antibody
    • Sodium/ hydrogen exchanger 8 antibody
    • Sodium/hydrogen exchanger 8 antibody
    • solute carrier family 9 (sodium/hydrogen exchanger), member 8 antibody
    • Solute carrier family 9 member 8 antibody
    see all

Anti-SLC9A8 antibody 图像

  • Anti-SLC9A8 antibody (ab83937) at 1 µg/ml (in 5% skim milk/ PBS buffer) + HepG2 cell lysate at 10 µg with gel concentration: 12%

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 46 kDa. We are unsure as to the identity of these extra bands.

Anti-SLC9A8 antibody (ab83937)参考文献

ab83937 has not yet been referenced specifically in any publications.

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