Anti-SLC6A7抗体(ab122024)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to SLC6A7
  • 经测试应用
    适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: SLTSDLPWEH CGNWWNTELC LEHRVSKDGN GALPLNLTCT VSPSEEYWSR YVLHIQGSQG IGSPG, corresponding to amino acids 147-211 of Human SLC6A7

  • 阳性对照
    • Human colon tissue

性能

应用

Our Abpromise guarantee covers the use of ab122024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Terminates the action of proline by its high affinity sodium-dependent reuptake into presynaptic terminals.
  • 组织特异性
    Brain.
  • 序列相似性
    Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A7 subfamily.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • solute carrier family 6 (neurotransmitter transporter, L-proline), member 7 antibody
    • brain-specific L-proline transporter antibody
    • L-proline transporter, brain-specific antibody
    • PROT antibody
    • SC6A7_HUMAN antibody
    • Slc6a7 antibody
    • Sodium-dependent proline transporter antibody
    • solute carrier family 6 (neurotransmitter transporter), member 7 antibody
    • Solute carrier family 6 member 7 antibody
    see all

图片

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm. Recommended concentration of ab122024 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122024, at a 1/20 dilution, staining SLC6A7 in paraffin-embedded Human colon tissue, by Immunohistochemistry.

文献

ab122024 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunocytochemistry
Sample
Human Cultured Cells (CHO)
Specification
CHO
Fixative
Formaldehyde
Permeabilization
Yes - 0.3% triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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提交于 May 18 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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