antigen sequence: SLTSDLPWEH CGNWWNTELC LEHRVSKDGN GALPLNLTCT VSPSEEYWSR YVLHIQGSQG IGSPG, corresponding to amino acids 147-211 of Human SLC6A7
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Terminates the action of proline by its high affinity sodium-dependent reuptake into presynaptic terminals.
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A7 subfamily.
Information by UniProt
- solute carrier family 6 (neurotransmitter transporter, L-proline), member 7 antibody
- brain-specific L-proline transporter antibody
- L-proline transporter, brain-specific antibody
Immunocytochemistry/ Immunofluorescence - Anti-SLC6A7 antibody (ab122024)
Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm. Recommended concentration of ab122024 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A7 antibody (ab122024)
ab122024, at a 1/20 dilution, staining SLC6A7 in paraffin-embedded Human colon tissue, by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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