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A 14-22 aa region within synthetic peptide: MASTVSPSTI AETPEPPPLS DHIRNAADIS VIVIYFLVVM AVGLWAMLKT , corresponding to N terminal amino acids 1-50 of Human SLC5A4. (Peptide available as ab81335 - SLC5A4 peptide (1-50).
Our Abpromise guarantee covers the use of ab60330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1:1562500.
ab60330 has not yet been referenced specifically in any publications.