概述

  • 产品名称Anti-SLC4A7抗体
    参阅全部 SLC4A7 一抗
  • 描述
    兔多克隆抗体to SLC4A7
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1150 to the C-terminus of Human SLC4A7.

    (Peptide available as ab96634.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: Caco2 and HEK293.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 136 kDa (predicted molecular weight: 136 kDa).

靶标

  • 相关性SLC4A7 regulates intracellular pH and may also play a role in bicarbonate salvage in secretory epithelia. Additionally, SLC4A7 may also have associated sodium channel activity.
  • 细胞定位Basolateral cell membrane; Multi pass membrane protein. Apical cell membrane; Multi pass membrane protein. Cell projection, stereocilium.
  • 数据库链接
  • 别名
    • Bicarbonate transporter antibody
    • BT antibody
    • NBC2 antibody
    • NBC3 antibody
    • S4A7 antibody
    • SBC2 antibody
    • SLC4A6 antibody
    • Sodium bicarbonate cotransporter 2 antibody
    • Sodium bicarbonate cotransporter 2b antibody
    • Sodium bicarbonate cotransporter 3 antibody
    • Solute carrier family 4 member 7 antibody
    • Solute carrier family 4 sodium bicarbonate cotransporter member 7 antibody
    see all

Anti-SLC4A7 antibody 图像

  • IHC image of SLC4A7 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82335, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • All lanes : Anti-SLC4A7 antibody (ab82335) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 10 µg
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 136 kDa
    Observed band size : 136 kDa
    Additional bands at : 19 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

Anti-SLC4A7 antibody (ab82335)参考文献

ab82335 has not yet been referenced specifically in any publications.

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