• 产品名称Anti-SLC4A4抗体
    参阅全部 SLC4A4 一抗
  • 描述
    兔多克隆抗体to SLC4A4
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human, Recombinant Fragment
    预测可用于: Rat
  • 免疫原

    Synthetic peptide corresponding to C terminal residues of human SLC4A4



Our Abpromise guarantee covers the use of ab56215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 120 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.


  • 功能Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.
  • 组织特异性Isoform 1 is expressed in pancreas and to a lower extent in heart, skeletal muscle, liver, parotid salivary glands, prostate, colon, stomach, thyroid, brain and spinal chord. Corneal endothelium cells express only isoform 1 (at protein level). Isoform 2 is specifically expressed in kidney at the level of proximal tubules.
  • 疾病相关Defects in SLC4A4 are the cause of proximal renal tubular acidosis with ocular abnormalities (pRTA-OA) [MIM:604278]; also known as renal tubular acidosis II. Caused by an impairment of bicarbonate absorption in the proximal tubule, proximal renal tubular acidosis (pRTA) is characterized by a decreased renal HCO3(-) threshold. pRTA-OA is an extremely rare autosomal recessive syndrome characterized by short stature, profound pRTA, mental retardation, bilateral glaucoma, cataracts and bandkeratopathy.
    Note=Loss of interaction with and stimulation by CA4 is the cause of retinitis pigmentosa type 17 (RP17).
  • 序列相似性Belongs to the anion exchanger (TC 2.A.31) family.
  • 翻译后修饰Phosphorylation of Ser-1026 by PKA increases the binding of CA2 and changes the Na(+):HCO3(-) stoichiometry of the transporter from 3:1 to 2:1. Phosphorylation of Thr-49 regulates isoform 1 conductance.
    N-glycosylation is not necessary for the transporter basic functions.
  • 细胞定位Basolateral cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp781H1314 antibody
    • Electrogenic sodium bicarbonate cotransporter 1 antibody
    • hhNMC antibody
    • HNBC 1 antibody
    • HNBC1 antibody
    • kNBC 1 antibody
    • KNBC antibody
    • kNBC1 antibody
    • Na(+)/HCO3(-) cotransporter antibody
    • Na+HCO3- cotransporter 4 antibody
    • NBC 1 antibody
    • NBC 2 antibody
    • NBC1 antibody
    • NBC2 antibody
    • Nbc4 antibody
    • NBCE 1 antibody
    • NBCE1 antibody
    • OTTHUMP00000160355 antibody
    • OTTHUMP00000218884 antibody
    • OTTHUMP00000218885 antibody
    • PNBC antibody
    • S4A4_HUMAN antibody
    • SLC4A4 antibody
    • SLC4A5 antibody
    • Sodium bicarbonate cotransporter 1 (sodium bicarbonate cotransporter, kidney; sodium bicarbonate cotransporter, pancreas) antibody
    • Sodium bicarbonate cotransporter antibody
    • Sodium bicarbonate cotransporter kidney antibody
    • sodium bicarbonate cotransporter member 4 antibody
    • Sodium bicarbonate cotransporter pancreas antibody
    • Solute carrier family 4 member 4 antibody
    • Solute carrier family 4 sodium bicarbonate cotransporter member 4 antibody
    • Solute carrier family 4 sodium bicarbonate cotransporter member 5 antibody
    • Solute carrier family 4, sodium bicarbonate cotransporter, member 4, brain type antibody
    see all

Anti-SLC4A4 antibody 图像

  • ICC/IF image of ab56215 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56215, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SLC4A4 antibody (ab56215)参考文献

ab56215 has not yet been referenced specifically in any publications.

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