概述

  • 产品名称
  • 描述
    兔多克隆抗体to SLC4A4
  • 经测试应用
    适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human, Recombinant fragment
    预测可用于: Rat
  • 免疫原

    Synthetic peptide corresponding to C terminal residues of human SLC4A4

性能

应用

Our Abpromise guarantee covers the use of ab56215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 120 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

靶标

  • 功能
    Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.
  • 组织特异性
    Isoform 1 is expressed in pancreas and to a lower extent in heart, skeletal muscle, liver, parotid salivary glands, prostate, colon, stomach, thyroid, brain and spinal chord. Corneal endothelium cells express only isoform 1 (at protein level). Isoform 2 is specifically expressed in kidney at the level of proximal tubules.
  • 疾病相关
    Defects in SLC4A4 are the cause of proximal renal tubular acidosis with ocular abnormalities (pRTA-OA) [MIM:604278]; also known as renal tubular acidosis II. Caused by an impairment of bicarbonate absorption in the proximal tubule, proximal renal tubular acidosis (pRTA) is characterized by a decreased renal HCO3(-) threshold. pRTA-OA is an extremely rare autosomal recessive syndrome characterized by short stature, profound pRTA, mental retardation, bilateral glaucoma, cataracts and bandkeratopathy.
    Note=Loss of interaction with and stimulation by CA4 is the cause of retinitis pigmentosa type 17 (RP17).
  • 序列相似性
    Belongs to the anion exchanger (TC 2.A.31) family.
  • 翻译后修饰
    Phosphorylation of Ser-1026 by PKA increases the binding of CA2 and changes the Na(+):HCO3(-) stoichiometry of the transporter from 3:1 to 2:1. Phosphorylation of Thr-49 regulates isoform 1 conductance.
    N-glycosylation is not necessary for the transporter basic functions.
  • 细胞定位
    Basolateral cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp781H1314 antibody
    • Electrogenic sodium bicarbonate cotransporter 1 antibody
    • hhNMC antibody
    • HNBC 1 antibody
    • HNBC1 antibody
    • kNBC 1 antibody
    • KNBC antibody
    • kNBC1 antibody
    • Na(+)/HCO3(-) cotransporter antibody
    • Na+HCO3- cotransporter 4 antibody
    • NBC 1 antibody
    • NBC 2 antibody
    • NBC1 antibody
    • NBC2 antibody
    • Nbc4 antibody
    • NBCE 1 antibody
    • NBCE1 antibody
    • OTTHUMP00000160355 antibody
    • OTTHUMP00000218884 antibody
    • OTTHUMP00000218885 antibody
    • PNBC antibody
    • S4A4_HUMAN antibody
    • SLC4A4 antibody
    • SLC4A5 antibody
    • Sodium bicarbonate cotransporter 1 (sodium bicarbonate cotransporter, kidney; sodium bicarbonate cotransporter, pancreas) antibody
    • Sodium bicarbonate cotransporter antibody
    • Sodium bicarbonate cotransporter kidney antibody
    • sodium bicarbonate cotransporter member 4 antibody
    • Sodium bicarbonate cotransporter pancreas antibody
    • Solute carrier family 4 member 4 antibody
    • Solute carrier family 4 sodium bicarbonate cotransporter member 4 antibody
    • Solute carrier family 4 sodium bicarbonate cotransporter member 5 antibody
    • Solute carrier family 4, sodium bicarbonate cotransporter, member 4, brain type antibody
    see all

图片

  • ICC/IF image of ab56215 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56215, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:

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客户评价及客户问答

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I have made a short list of compatible antibodies for use withab56215 Anti-SLC4A4 antibody.These products are pre-absorbed to ensure minimum cross reactivity. Each product is ...

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Thank you for contacting us and your interest in our products. The immunogen sequence used to raise theab56215 Anti-SLC4A4 antibodyis proprietary information. However, in order to aid your research I am able to share it with you. Please do not share...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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