Anti-MATE-1抗体(ab92295)
Key features and details
- Goat polyclonal to MATE-1
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MATE-1抗体
参阅全部 MATE-1 一抗 -
描述
山羊多克隆抗体to MATE-1 -
宿主
Goat -
经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human MATE-1 aa 528-541 (internal sequence) (Cysteine residue).
Sequence:C-PEHPQDGAKLSRKQ
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阳性对照
- WB: Human liver tissue lysate; ICC: HepG2 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92295于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 62 kDa.
1 hour primary incubation is recommended for this product. |
说明 |
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ICC
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 62 kDa. 1 hour primary incubation is recommended for this product. |
靶标
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功能
Solute transporter for tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, N-methylnicotinamide (NMN), metformin, creatinine, guanidine, procainamide, topotecan, estrone sulfate, acyclovir, ganciclovir and also the zwitterionic cephalosporin, cephalexin and cephradin. Seems to also play a role in the uptake of oxaliplatin (a new platinum anticancer agent). Able to transport paraquat (PQ or N,N-dimethyl-4-4'-bipiridinium); a widely used herbicid. Responsible for the secretion of cationic drugs across the brush border membranes. -
组织特异性
Expressed in adrenal gland, and to a lower extent in liver, skeletal muscle and kidney (especially found in luminal membranes of the urinary tubules, bile caniculi and brush border membranes). -
序列相似性
Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. -
细胞定位
Cell membrane. Predominantly localized at the plasma membrane but also found in intracellular organelles. - Information by UniProt
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数据库链接
- Entrez Gene: 55244 Human
- Omim: 609832 Human
- SwissProt: Q96FL8 Human
- Unigene: 232054 Human
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别名
- FLJ10847 antibody
- hMATE-1 antibody
- hMATE1 antibody
see all
图片
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Immunocytochemistry analysis of paraformaldehyde fixed HepG2 cells labeling MATE-1. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
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All lanes : Anti-MATE-1 antibody (ab92295) at 0.1 µg/ml
Lane 1 : Human liver lysate (in RIPA buffer)
Lane 2 : Human liver lysate (in RIPA buffer) with immunizing peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 62 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
数据表及文件
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Datasheet download
文献 (0)
ab92295 尚未被引用在任何文献中。