Synthetic peptide corresponding to a region within N terminal amino acids 1-50 of Human SLC46A3 (MKILFVEPAI FLSAFAMTLT GPLTTQYVYR RIWEETGNYT FSSDSNISEC)
Jurkat cell lysate. Human kidney.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2.5 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
Use at an assay dependent concentration.
Titre using peptide based assay: 1/62500.
SLC46A3 (Solute carrier family 46 member 3) is a member of the major facilitator superfamily and SLC46A family. Its function remains unknown.
Membrane; Multi-pass membrane protein.
FKSG 16 antibody
Western blot - Anti-SLC46A3 antibody (ab62301)
All lanes :
SLC46A3 antibody (ab62301) at 2.5µg/ml (in 5% skim milk / PBS buffer)
Lane 1 :
Molecular weight marker
Lane 2 :
Jurkat cell lysate at 10 µg
Secondary All lanes :
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size:
Observed band size:
44 kDa (
why is the actual band size different from the predicted?
Additional bands at:
10 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC46A3 antibody (ab62301)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SLC46A3 with ab62301 at 4-8µg/ml. Arrows indicate positively stained epithelial cells of the renal tubule. Magnification: 400X.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"