Rabbit, Horse, Dog
A region within synthetic peptide: MAGQGLPLHV ATLLTGLLEC LGFAGVLFGW PSLVFVFKNE DYFKDLCGPD, corresponding to N terminal amino acids 1-50 of Human SLC43A3
- Jurkat cells; Lung tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent concentration.
Titre using peptide based assay: 1:1562500.
||Use a concentration of 2.5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use a concentration of 4 - 8 µg/ml.
SLC43A3 is a putative transporter, belonging to the SLC43A transporter family. It is highly expressed in macrophages.
Multi-pass membrane protein
- DKFZp762A227 antibody
- EEG1 antibody
- FOAP 13 antibody
Western blot - Anti-SLC43A3 antibody (ab60302)
All lanes :
Anti-SLC43A3 antibody (ab60302) at 2.5 µg/mlLane 1 :
Molecular weight markerLane 2 :
Jurkat cell lysate at 10 µgSecondaryAll lanes :
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
55 kDaObserved band size:
48 kDa (why is the actual band size different from the predicted?
Gel concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC43A3 antibody (ab60302)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling SLC43A3 with ab60302 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.
has not yet been referenced specifically in any publications.
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