概述

  • 产品名称
    Anti-SLC41A1抗体
  • 描述
    兔多克隆抗体to SLC41A1
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    A synthetic peptide corresponding to a region within the N-terminal sequence 35-84 (TSEFLGPDGAGVEVVIESRANAKGVREEDALLENGSQSNESDDVSTDRG P) of Human SLC41A1 (NP_776253).

  • 阳性对照
    • 721_B Cell Lysate

性能

应用

Our Abpromise guarantee covers the use of ab83701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

靶标

Anti-SLC41A1 antibody 图像

  • Anti-SLC41A1 antibody (ab83701) at 1 µg/ml (diluted in 5% skim milk / PBS buffer) + 721_B Cell Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

实验方案

Anti-SLC41A1 antibody (ab83701)参考文献

This product has been referenced in:
  • Mandt T  et al. SLC41A1 Mg(2+) transport is regulated via Mg(2+)-dependent endosomal recycling through its N-terminal cytoplasmic domain. Biochem J 439:129-39 (2011). Read more (PubMed: 21696366) »

See 1 Publication for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Whole embryo 6 days post fertilization)
Gel Running Conditions
Non-reduced Denaturing (10 % SDS PAGE)
Loading amount
6000 µg
Specification
Whole embryo 6 days post fertilization
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Greg Bonde

Verified customer

提交于 Aug 14 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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