概述

  • 产品名称Anti-SLC38A4抗体
  • 描述
    兔多克隆抗体to SLC38A4
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • 免疫原

    A region within synthetic peptide: RRKMQTVSNI SITGMLVMYL LAALFGYLTF YGEVEDELLH AYSKVYTLDI, corresponding to C terminal amino acids 361-410 of Human SLC38A4

  • 阳性对照
    • Jurkat cell lysate; human kidney tissue

性能

应用

Our Abpromise guarantee covers the use of ab58785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 61 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 相关性SLC38A4 is found predominantly in liver and transports both cationic and neutral amino acids. The transport of cationic amino acids by SLC38A4 is Na+ and pH independent, while the transport of neutral amino acids is Na+ and pH dependent. It has a broad specificity, with a preference for Ala, followed by His, Cys, Asn, Ser, Gly, Val, Thr, Gln and Met.
  • 细胞定位Cell Membrane; Multi-pass membrane protein
  • 数据库链接
  • 别名
    • Amino acid transporter A3 antibody
    • Na(+)-coupled neutral amino acid transporter 4 antibody
    • Sodium-coupled neutral amino acid transporter 4 antibody
    • Solute carrier family 38 member 4 antibody
    • System N amino acid transporter 3 antibody
    see all

Anti-SLC38A4 antibody 图像

  • Lane 1 : marker
    Lane 2 : Anti-SLC38A4 antibody (ab58785) at 2.5 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg with 5% skim milk in PBS buffer

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 61 kDa
    Gel concentration 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SLC38A4 with ab58785 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400X.

Anti-SLC38A4 antibody (ab58785)参考文献

This product has been referenced in:
  • Desforges M  et al. The SNAT4 isoform of the system A amino acid transporter is functional in human placental microvillous plasma membrane. J Physiol 587:61-72 (2009). WB, IHC-P ; Human . Read more (PubMed: 19015196) »

See 1 Publication for this product

Product Wall



Unfortunately, at this time, the only data our lab has is the western blot and IHC data with human samples. We have not conducted additional experiments to address why the staining seems to be mainly cytoplasmic. Additionally, from the 14aa p...

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