概述

  • 产品名称Anti-SLC38A2抗体
  • 描述
    兔多克隆抗体to SLC38A2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee, Spider Monkey
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (ALKSHYADVD PENQNFLLES NLGKKKYETE FHPGTTSFGM SVFNLSNAIV) of Human SLC38A2 (NP_061849).

  • 阳性对照
    • HT1080 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab90677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Functions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of neutral amino acids and sodium ions with a stoichiometry of 1:1. May function in the transport of amino acids at the blood-brain barrier and in the supply of maternal nutrients to the fetus through the placenta.
  • 组织特异性Ubiquitously expressed. Widely expressed in the central nervous system with higher concentrations in caudal regions. Expressed by glutamatergic and GABAergic neurons together with astrocytes and other non-neuronal cells in the cerebral cortex (at protein level).
  • 序列相似性Belongs to the amino acid/polyamine transporter 2 family.
  • 翻译后修饰Polyubiquitination by NEDD4L regulates the degradation and the activity of SLC38A2.
  • 细胞定位Cell membrane. Insulin promotes recruitment to the plasma membrane from a pool localized in the trans-Golgi network or endosomes (By similarity). Enriched in the somatodendritic compartment of neurons, it is also detected at the axonal shaft but excluded from the nerve terminal.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Amino acid transporter 2 antibody
    • Amino acid transporter A2 antibody
    • ATA2 antibody
    • KIAA1382 antibody
    • PRO1068 antibody
    • Protein 40-9-1 antibody
    • S38A2_HUMAN antibody
    • SAT2 antibody
    • Slc38a2 antibody
    • SNAT2 antibody
    • Sodium-coupled neutral amino acid transporter 2 antibody
    • Solute carrier family 38 member 2 antibody
    • System A amino acid transporter antibody
    • System A amino acid transporter 2 antibody
    • System A transporter 1 antibody
    • System N amino acid transporter 2 antibody
    see all

Anti-SLC38A2 antibody 图像

  • Anti-SLC38A2 antibody (ab90677) at 1 µg/ml (in 5% skim milk / PBS buffer) + HT1080 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 56 kDa

Anti-SLC38A2 antibody (ab90677)参考文献

This product has been referenced in:
  • Oh RS  et al. Functional RNA interference (RNAi) screen identifies system A neutral amino acid transporter 2 (SNAT2) as a mediator of arsenic-induced endoplasmic reticulum stress. J Biol Chem 287:6025-34 (2012). WB ; Human, Mouse . Read more (PubMed: 22215663) »

See 1 Publication for this product

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Here is a link to our subcellular fractionation protocol

http://www.abcam.com/index.html?pageconfig=resource&rid=11473

We also have a plasma membrane protein extraction kit (ab65400) t...

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Please be aware that we do not optimise our results when we test our antibodies: we either see the predicted band, or we don't. In addition, the predicted band must be at clearly distinguishable from other side b...

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (placenta, day 20)
Loading amount 30 µg
Specification placenta, day 20
Gel Running Conditions Reduced Denaturing (5%)
Blocking step (agent) for 1 minute(s) · Concentration: 1µg/mL
Username

Dr. Heidi Engelhardt

Verified customer

提交于 Feb 26 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"